Arctigenin attenuates paraquat-induced human lung epithelial A549 cell injury by suppressing ROS/p38 mitogen-activated protein kinases-mediated apoptosis  

作　　者：Chao Liu Zhao-rui Sun Meng-meng Wang Zhi-zhou Yang Wei Zhang Yi Ren Xiao-qin Han Rui Liu Quan Li Shi-nan Nie 

机构地区：[1]Department of Emergency Medicine,Jinling Hospital,Medical School of Nanjing University,Nanjing 210002,China

出　　处：《World Journal of Emergency Medicine》2022年第5期373-378,共6页世界急诊医学杂志（英文）

基　　金：This work was supported by the National Natural Science Foundation of China(82172182 and 82102311);Social Development Projects of Jiangsu Province(BE2017720);Natural Science Foundation of Jiangsu Province(BK20190247);Science Foundation of Jiangsu Health Commission(H2018039);Jiangsu Postdoctoral Research Foundation(2018K048A and 2020Z193).

摘　　要：BACKGROUND:Paraquat(PQ)-induced acute lung injury(ALI)and pulmonary fi brosis are common diseases with high mortality but without eff ective antidotes in emergency medicine.Our previous study has proved that arctigenin suppressed pulmonary fibrosis induced by PQ.We wondered whether arctigenin could also have a protective eff ect on PQ-induced ALI.METHODS:A PQ-induced A549 cell injury model was used,and the effect of arctigenin was determined by a cell counting kit-8(CCK-8)cell viability assay.In addition,terminal deoxynucleotidyl transferase(TdT)-mediated dUTP nick-end labelling(TUNEL)staining assays and mitochondrial membrane potential assays were performed to evaluate the level of cell apoptosis.The generation of reactive oxygen species(ROS)was refl ected by dihydroethidium(DHE)staining and a 2’,7’-dichlorodihy drofluorescein diacetate(DCFH-DA)assay.Moreover,immunoblotting studies were used to assess the expression of mitogen-activated protein kinases(MAPKs)and p38 MAPK.RESULTS:Arctigenin attenuated PQ-induced inhibition of A549 cell viability in a dose-dependent manner.Arctigenin also significantly reduced PQ-induced A549 cell apoptosis,as refl ected by the TUNEL assay and mitochondrial membrane potential assay,which may result from suppressed ROS/p38 MAPK signaling because we found that arctigenin dramatically suppressed ROS generation and p38 MAPK phosphorylation.CONCLUSION:Arctigenin could attenuate PQ-induced lung epithelial A549 cell injury in vitro by suppressing ROS/p38 MAPK-mediated cell apoptosis,and arctigenin might be considered a potential candidate drug for PQ-induced ALI.

关 键 词：PARAQUAT A549 cells ARCTIGENIN Reactive oxygen species Mitogen-activated protein kinases Apoptosis 

分 类 号：R285[医药卫生—中药学]