金丝桃苷-环糊精包合物的制备及其抗氧化活性研究  

Preparation of Hyperoside-Cyclodextrin Inclusion Complex and Its Biological Antioxidant Activity

作　　者：张馨予 苏建青[1] 褚秀玲[1] ZHANG Xinyu;SU Jianqing;CHU Xiuling(College of Agronomy and Agricultural Engineering,Liaocheng University,Liaocheng 252000,China)

机构地区：[1]聊城大学农学与农业工程学院,聊城252000

出　　处：《动物营养学报》2022年第8期5426-5440,共15页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金(31872515,32172901)。

摘　　要：金丝桃苷是一种水溶性低的黄酮类物质,为了提高其水溶性、稳定性和抗氧化活性,本文选择3种适宜的环糊精(羟乙基-β-环糊精、β-环糊精和甲基-β-环糊精)对其进行包合。试验应用扫描电子显微镜、X-射线衍射、薄层色谱、热重分析及溶解性试验等方法,对超声波法制备金丝桃苷的3种环糊精包合物进行表征分析以及溶解度测定;在此基础上,通过体外抗氧化和细胞氧化应激模型比较了包合前后的金丝桃苷抗氧化活性。结果表明:金丝桃苷与3种环糊精的包合比均为摩尔比1∶1。扫描电子显微镜、X-射线衍射及薄层色谱等表征均显示金丝桃苷和环糊精形成了包合物。其中,金丝桃苷-羟乙基-β-环糊精包合物的溶解度提高了8.2倍,具有最高的的溶解度。体外抗氧化和细胞氧化应激试验结果显示,金丝桃苷对2,2-二苯基-1-苦基肼(DPPH)和2,2′氨基-二(3-乙基-苯并噻唑啉磺酸-6)铵盐(ABTS)自由基清除的半抑制浓度(IC50)分别为7.136和8.251μg/mL,而金丝桃苷-羟乙基-β-环糊精包合物的IC50分别为6.500和6.362μg/mL。另外,与金丝桃苷组相比,中浓度(100μg/mL)金丝桃苷-羟乙基-β-环糊精包合物组细胞丙二醛(MDA)含量极显著降低(P<0.01),细胞超氧化物歧化酶(SOD)活性显著提高(P<0.05)。由此可见,羟乙基-β-环糊精作为金丝桃苷的包合材料,能提供最好的溶解度,提高金丝桃苷-羟乙基-β-环糊精包合物的体外抗氧化活性。Hyperoside was a kind of flavonoid with low water solubility,in order to improve its water solubility,stability and antioxidant activity,three suitable cyclodextrins(hydroxyethyl-β-cyclodextrin,β-cyclodextrin and methyl-β-cyclodextrin)were selected to coat it.The scanning electron microscope,X-ray diffraction,thin layer chromatography,thermogravimetric analysis and solubility test were used to characterize the three cyclodextrin inclusion complexes of hyperoside prepared by ultrasonic method.On this basis,the antioxidant activity of hyperoside before and after inclusion were compared by in vitro antioxidant and cellular oxidative stress models.The results showed that the inclusion ratio of hyperoside to three kinds of cyclodextrins was mole ratio 1∶1.The scanning electron microscope,X-ray diffraction and thin layer chromatography showed that hyperoside and cyclodextrin formed inclusion complexes.Among them,the solubility of hyperoside-hydroxyethyl-β-cyclodextrin inclusion complex increased 8.2 times,and it had the highest solubility.The in vitro antioxidant and cellular oxidative stress tests showed that the 50%inhibiting concentration(IC50)of hyperoside on 2,2-diphenyl-1-picrylhydrazyl(DPPH)and 2,2′-amino-di(2-ethyl-benzothiazoline sulphonic acid-6)ammonium salt(ABTS)free radical scavenging were 7.136 and 8.251μg/mL,respectively,while the IC50of hyperoside-hydroxyethyl-β-cyclodextrin inclusion complex were 6.500 and 6.362μg/mL,respectively.In addition,compared with hyperoside group,the cell malondialdehyde(MDA)content of medium concentration(100μg/mL)hyperoside-hydroxyethyl-β-cyclodextrin inclusion complex group was extremely significantly decreased(P<0.01),and the cell superoxide dismutase(SOD)activity was significantly increased(P<0.05).In conclusion,the hydroxyethyl-β-cyclodextrin as hyperoside coating material can provide the best solubility,and improve the in vitro antioxidant activity of hyperoside-hydroxyethyl-β-cyclodextrin inclusion complex.

关 键 词：金丝桃苷 包合物 抗氧化活性 

分 类 号：S816.7[农业科学—饲料科学]