深圳市大鹏新区2起儿童疱疹性咽颊炎聚集性疫情的病原鉴定与基因分型分析  

Pathogen identification and genotyping of two cluster outbreaks of herpangina in children in Dapeng New District,Shenzhen

作　　者：黄妙芬 王文祥 连宪强 杨粤 HUANG Miaofen;WANG Wenxiang;LIAN Xianqiang;YANG Yue(Dapeng New District Center for Disease Control and Prevention,Shenzhen,Guangdong 518116,China)

机构地区：[1]深圳市大鹏新区疾病预防控制中心,广东深圳518116

出　　处：《上海预防医学》2022年第7期660-664,共5页Shanghai Journal of Preventive Medicine

基　　金：广东省深圳市大鹏新区科技创新和产业发展专项资金(YL202001-02)。

摘　　要：【目的】分析2021年5月深圳市大鹏新区2起儿童疱疹性咽颊炎疫情的病原特征及其基因型。【方法】采集2起疱疹性咽峡炎暴发疫情患儿的5份咽拭子,用实时荧光聚合酶链式反应(PCR)对其进行常见肠道病毒的基因检测,采用逆转录巢式聚合酶链式反应(RT⁃nPCR)法扩增肠道病毒VP1区,通过测序和比对进行序列分析,运用MEGA7软件中的CLUSTAL W程序进行位点对齐并构建系统进化树。【结果】引起这2起疱疹性咽峡炎疫情的病原体为柯萨奇病毒A4(CVA4)。VP1区基因序列分析发现2起疫情样本之间的核苷酸同源性为92%;A幼儿园的3株毒株与深圳2018株(MN840533)进化关系最近,核苷酸同源性为98.11%;B幼儿园的2株毒株与四川2018株(MW178763)进化关系最近,核苷酸同源性为97.88%。系统进化树显示,本研究的5株CVA4病毒均属C2基因亚型。【结论】2起儿童疱疹性咽颊炎疫情都是由CVA4病毒引起,均属C2基因亚型。[Objective]To determine the pathogenic characteristics and genotype of two outbreaks of herpangina in children in Dapeng New District,Shenzhen,in May 2021.[Methods]A total of five throat swabs from children in the two outbreaks of herpangina were collected and examined for common enteroviruses by real-time PCR.The VP1 region was further amplified by nested RT-PCR.The CLUSTAL W program in MEGA7 software was used to conduct the alignment and reconstruct a phylogenetic tree.[Results]The pathogen causing the 2 cluster outbreaks of herpangina was coxsackievirus A4(CVA4).The sequences of CVA4 VP1 genes revealed that a nucleotide identity of 92%between the strains in the two outbreaks.The three CVA4 strains isolated in kindergarten A had the closest phylogenetic relationship with that isolated in Shenzhen in 2018(MN840533),with the nucleotide identity of 98.11%.The two strains in kindergarten B had the closest phylogenetic relationship with CVA4 strain isolated in Sichuan in 2018(MW178763),with the nucleotide identity of 97.88%.The phylogenetic tree showed that all five CVA4 strains in this study belonged to the C2 genotype.[Conclusion]The C2 genotype of CVA4 is the causative agent in both outbreaks of herpangina.

关 键 词：柯萨奇病毒A4 疱疹性咽颊炎 聚集性疫情 基因型 基因序列 系统进化树 

分 类 号：R511[医药卫生—内科学]