皮肤致敏性替代试验方法的研究进展  

Research Progress on Alternative Methods of Skin Sensitization Test

作　　者：黄静怡 李培宁 刘香梅 刘忠华[1] 黄宇锋 HUANG Jingyi;LI Peining;LIU Xiangmei;LIU Zhonghua;HUANG Yufeng(College of Veterinary Medicine of South China Agricultural University,Guangzhou 510630,China;Guangzhou Quality Supervision and Testing Institute,Guangzhou 511447,China)

机构地区：[1]华南农业大学兽医学院,广州510630 [2]广州质量监督检测研究院,广州511447

出　　处：《实验动物与比较医学》2022年第4期313-321,共9页Laboratory Animal and Comparative Medicine

摘　　要：过敏性接触性皮炎是皮肤反复接触某种物质后所引起的Ⅳ型超敏反应,也是常见的公共健康问题。传统皮肤致敏性测试以豚鼠最大值试验、封闭斑贴等动物实验为主。近年来,随着动物伦理不断被人们重视以及科学技术的发展,皮肤致敏试验替代方法相继出现。根据原理不同,这些替代方法分为体内替代法、几类基于有害结局通路的体外替代法、基因组过敏原快速检测法等。本文将对这些皮肤致敏试验替代方法的研究进展以及几类基于有害结局通路的整合测试与评估方法进行综述。Allergic contact dermatitis is a typeⅣhypersensitivity reaction caused by repeated skin exposure to a substance and is a common public health problem.Traditional skin sensitization tests are based on animal experiments such as guinea pig maximum test and closed patch.In recent years,with the increasing attention of animal ethics and the development of science and technology,alternative methods of skin sensitization test have emerged.According to different principles,these alternative methods are divided into in vivo alternative methods,several in vitro alternative methods based on harmful outcome pathways,and genomic allergen rapid test,etc.In this paper,we reviewed the progress of these alternative methods of skin sensitization test,and several integrated testing and evaluation methods based on adverse outcome pathways.

关 键 词：皮肤致敏性试验 过敏性接触性皮炎 替代方法 

分 类 号：R-332[医药卫生] Q95-33[生物学—动物学]