欧李组培快繁培养基的筛选  

Screening of tissue culture and rapid propagation medium of Cerasus humilis

作　　者：袁振 廖荣君 乔福利 冯丽花 辛欣 冯殿齐 YUAN Zhen;LIAO Rongjun;QIAO Fuli;FENG Lihua;XIN Xin;FENG Dianqi(Shandong Yiteng New Materials Co.,Ltd.,Tai’an,Shandong 271000,China;Shandong Yidelai Biotechnology Co.,Ltd.,Tai’an,Shandong 271000,China;Shandong Taishanyuan Agricultural Technology Co.,Ltd.,Tai’an,Shandong 271000,China;Tai’an Landscaping Management Service Center,Tai’an,Shandong 271000,China;Taishan Scenic Spot Management Committee,Tai’an,Shandong 271000,China)

机构地区：[1]山东一滕新材料股份有限公司,山东泰安271000 [2]山东益得来生物科技有限公司,山东泰安271000 [3]山东泰山源农业科技有限公司,山东泰安271000 [4]泰安市园林绿化管理服务中心,山东泰安271000 [5]泰山风景名胜区管理委员会,山东泰安271000

出　　处：《落叶果树》2022年第4期32-37,共6页Deciduous Fruits

摘　　要：以4个欧李优系的嫩枝顶芽及带叶腋的茎段作外植体进行组培快繁,研究不同培养阶段的适宜培养基。结果表明,优系TSO-4的初代培养基为MS+0.5 mg/L 6-BA+0.1 mg/L NAA,继代培养基MS+0.5 mg/L 6-BA+0.1 mg/L NAA,生根培养基1/2MS+0.1 mg/L NAA+0.4 g/L活性炭;优系TSO-5的初代培养基为MS+0.5 mg/L 6-BA+0.1 mg/L NAA,继代培养基MS+0.5 mg/L 6-BA+0.1 mg/L NAA,生根培养基1/2MS+0.05 mg/L NAA;优系TSO-7的初代培养基为MS+0.5 mg/L 6-BA+0.1 mg/L NAA,继代培养基MS+0.3 mg/L 6-BA+0.1 mg/L NAA,生根培养基1/8MS+0.05 mg/L IAA;优系TSO-11的初代培养基为MS+0.5 mg/L 6-BA+0.1 mg/L NAA,继代培养基MS+0.5 mg/L 6-BA+0.1 mg/L NAA,生根培养基1/2MS+0.1 mg/L NAA;4个欧李优系的炼苗基质均为草炭土∶珍珠岩=1∶2。Tissue culture and rapid propagation of Cerasus humilis stem segments were studied in this paper.The result shows that the best formula for primary culture with the stem segments as explants was MS+0.5 mg/L 6-BA+0.1 mg/L NAA,the best formula for subculture was MS+0.5 mg/L 6-BA+0.1 mg/L NAA,and the best formula for rooting culture was 1/2 MS+0.1 mg/L NAA+0.4 g/L activated carbon for TSO-4,the best formula for primary culture with the stem segments as explants was MS+0.5 mg/L 6-BA+0.1 mg/L NAA,the best formula for subculture was MS+0.5 mg/L 6-BA+0.1 mg/L NAA,and the best formula for rooting culture was 1/2MS+0.1 mg/L NAA for TSO-5,the best formula for primary culture with the stem segments as explants was MS+0.5 mg/L 6-BA+0.1 mg/L NAA,the best formula for subculture was MS+0.5 mg/L 6-BA+0.1 mg/L NAA,and the best formula for rooting culture was 1/8MS+0.1mg/L IAA for TSO-7,the best formula for primary culture with the stem segments as explants was MS+0.5 mg/L 6-BA+0.1 mg/L NAA,the best formula for subculture was MS+0.5 mg/L 6-BA+0.1 mg/L NAA,and the best formula for rooting culture was 1/2MS+0.1 mg/L NAA for TSO-11,and the best culturing matrix was peat soil+perlite(1∶2)for the four superior lines of Cerasus humilis.

关 键 词：欧李 组培 快速繁殖 培养基 

分 类 号：S662.3[农业科学—果树学]