HPV检测合人髓细胞增生原癌基因检测对宫颈癌前病变筛查的临床价值  

The Clinical Value of HPV Detection Combined with Human Myeloid Hyperplasia Proto-oncogene Detection in the Screening of Cervical Precancerous Lesions

作　　者：青措吉 张文静 吴德慧[1] 崔英[1] 丁鑫[1] QING Cuo-ji;ZHANG Wen-jing;WU De-hui;CUI Ying;DING Xin(Department of Gynaecology,Qinghai Red Cross Hospital,Xining,Qinghai,810000,China;Medical College,Qinghai university,Xining,Qinghai,810016,China)

机构地区：[1]青海红十字医院妇科,青海西宁810000 [2]青海大学医学院,青海西宁810016

出　　处：《现代生物医学进展》2022年第13期2587-2591,共5页Progress in Modern Biomedicine

基　　金：青海省卫健委科研项目(2019-wjyd-09)。

摘　　要：目的:探讨人乳头状瘤病毒(HPV)检测联合人髓细胞增生原癌基因(C-MYC)检测对宫颈癌前病变-宫颈上皮内瘤变(CIN)筛查的临床价值。方法:选择2018年6月至2020年12月在本院妇科保存的宫颈上皮内瘤变标本140份,采用免疫组化法检测C-MYC表达情况,采用PCR检测HPV16、HPV18表达情况并进行相关性分析。结果:在140份标本中,HPV16与HPV18的阳性率为62.9%与61.4%;CIN 1级标本HPV16、HPV18阳性率分别为26.8%和24.4%,2级标本分别为62.7%和58.8%,3级标本分别为93.8%和95.8%,对比有差异(P<0.05)。C-MYC阳性率为83.6%,不同CIN分级程度的标本组织中C-MYC阳性率对比有差异(P<0.05)。Spearsman相关分析显示HPV16、HPV18、C-MYC阳性率与CIN分级呈相关性(P<0.05)。二分类Logisitc回归分析显示HPV16、HPV18、C-MYC阳性率都为影响CIN分级的重要危险因素(P<0.05)。结论:随着宫颈上皮内瘤变级别的增加,HPV16、HPV18的阳性率也在升高,同时伴随C-MYC的过表达,两者具有相关性,是导致患者病情加重的重要危险因素。Objective:To explore the clinical values of human papillomavirus(HPV) detection combined with human myelocytomatosis oncogene(C-MYC) detection in the screening of cervical precancerous lesions-cervical intraepithelial neoplasia(CIN).Methods:A total of 140 specimens of cervical squamous intraepithelial lesions were selected from the department of our hospital from June 2018 to December 2020,the expression of C-MYC were detected by immunohistochemistry,the expression of HPV16,HPV18were detected by PCR and given correlation analysis.Results:In the 140 specimens,the positive rates of HPV16 and HPV18 were 62.9 %,61.4 %;the positive rates of HPV16 and HPV18 were 26.8 %,24.4 % in CIN level 1 specimens,and 62.7 %,58.8 % in level 2specimens,and 93.8 %,95.8 % in level 3 specimens,respectively,and compared the difference were statistically significant(P<0.05).The positive rates of C-MYC were 83.6 %;with the increase in the degree of CIN classification,the positive rates of C-MYC in the specimen tissue were also increased,and compared the difference were statistically significant(P<0.05).Spearsman correlation analysis showed that the positive rates of HPV16,HPV18,C-MYC were all correlated with CIN level(P<0.05).Binary Logisitc regression analysis showed that the positive rates of HPV16,HPV18,C-MYC were all important risk factors affect CIN level(P<0.05).Conclusion:With the increase of the grade of cervical intraepithelial neoplasia,the positive rate of HPV16 and HPV18 also increased,accompanied by the overexpression of C-My C,which were correlated and were important risk factors leading to the aggravation of patients’ disease.

关 键 词：宫颈上皮内瘤变 人乳头状瘤病毒 人髓细胞增生原癌基因 宫颈癌 相关性 

分 类 号：R737.33[医药卫生—肿瘤]