lncRNA UCA1通过miR-513a-3p/CYP1B1生物学轴促进人肺癌细胞增殖和迁移的机制研究  

Mechanism of lncRNA UCA1 promoting the proliferation and migration of human lung cancer cells through miR-513a-3p/CYP1B1 biological axis

作　　者：徐绍娜 徐晓焱[1] 祝慧慧[1] 马山[1] 张淑芹[1] 周晶琳[1] Xu Shaona;Xu Xiaoyan;Zhu Huihui(Biotechnology Laboratory,Mudanjiang Medical College,Mudanjiang 157000)

机构地区：[1]牡丹江医学院生物技术实验室,黑龙江牡丹江157000

出　　处：《中国现代医药杂志》2022年第8期13-18,共6页Modern Medicine Journal of China

摘　　要：目的研究长链非编码RNA UCA1(lncRNA UCA1)在肺癌细胞中的表达水平,并探讨UCA1通过microRNA-513a-3p(miR-513a-3p)和CYP1B1分子轴对肺癌细胞增殖和迁移的影响。方法通过定量PCR(qPCR)检测UCA1、miR-513a-3p和CYP1B1基因的表达,CCK8试剂盒检测细胞增殖活性,Transwell小室检测细胞迁移能力;向肺癌细胞中转入小干扰RNA(si-UCA1)敲低肺癌细胞UCA1表达(si-NC作为阴性对照),转入miR-513a-3p模拟物(mimic)上调肺癌细胞miR-513a-3p表达(NC作为阴性对照),转入miR-513a-3p抑制剂(inhibitor)下调肺癌细胞miR-513a-3p表达。结果与人正常肺上皮细胞(BEAS-2B)相比,UCA1在不同肺癌细胞(A549、H1299、NCI-H3255、NCI-H460和NCI-H838)中表达均显著升高(P<0.05),并且增殖和迁移能力均显著升高(P<0.05),且随UCA1表达升高而升高。双荧光素酶基因报告实验显示,miR-513a-3p与UCA1和CYP1B1靶向结合。与si-NC组相比,si-UCA1显著上调miR-513a-3p和下调UCA1在肺癌细胞中的表达(P<0.05);与NC组相比,mimic显著下调肺癌细胞中UCA1和CYP1B1表达(P<0.05),inhibitor显著上调肺癌细胞中UCA1和CYP1B1表达(P<0.05)。与si-UCA1组相比,共转入siUCA1和inhibitor可以显著提高因转入si-UCA1而降低的CYP1B1表达和肺癌细胞的增殖、迁移能力。结论LncRNA UCA1通过miR-513a-3p/CYP1B1生物学轴促进人肺癌细胞的增殖和迁移。Objective To study the expression level of long-chain non-coding RNA UCA1(lncRNA UCA1)in lung cancer cells,and to explore the effect of UCA1 on the proliferation and migration of lung cancer cells through microRNA-513a-3p(miR-513a-3p)and CYP1B1 molecular axis.Methods qPCR was used to detect the expression of UCA1,miR-513a-3p and CYP1B1,CCK8 was used to assess the cell viability and Transwell chamber was used to evaluate cell migration.Results Compared with human normal lung epithelial cells(BEAS-2B),UCA1 expression in different lung cancer cells(A549,H1299,NCI-H3255,NCI-H460 and NCI-H838)were significantly increased(P<0.05),the proliferation and migration ability were significantly increased(P<0.05).The double luciferase gene report experiment showed that miR-513a-3p bind to UCA1 and CYP1B1.Compared with the si-NC group,si-UCA1 significantly up-regulated miR-513a-3p and down-regulated the expression of UCA1 in lung cancer cells(P<0.05).Compared with the NC group,mimic significantly down-regulated the expression of UCA1 and CYP1B1 in lung cancer cells(P<0.05),inhibitor significantly up-regulated the expression of UCA1 and CYP1B1 in lung cancer cells(P<0.05).Compared with the si-UCA1 group,co-transfer of si-UCA1 and inhibitor could significantly increase the expression of CYP1B1 and the proliferation and migration ability of lung cancer cells.Conclusion LncRNA UCA1 promote the proliferation and migration of human lung cancer cells through miR-513a-3p/CYP1B1 biological axis.

关 键 词：肺癌 UCA1 miR-513a-3p CYP1B1 

分 类 号：R734.2[医药卫生—肿瘤]