Enhanced tissue regeneration through immunomodulation of angiogenesis and osteogenesis with a multifaceted nanohybrid modified bioactive scaffold  

作　　者：Hang Xue Zhenhe Zhang Ze Lin Jin Su Adriana CPanayi Yuan Xiong Liangcong Hu Yiqiang Hu Lang Chen Chenchen Yan Xudong Xie Yusheng Shi Wu Zhou Bobin Mi Guohui Liu 

机构地区：[1]Department of Orthopaedics,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,430022,China [2]Hubei Province Key Laboratory of Oral and Maxillofacial Development and Regeneration,Wuhan,430022,China [3]State Key Laboratory of Materials Processing and Die&Mould Technology,School of Materials Science and Engineering,Huazhong University of Science and Technology,Wuhan,430074,China [4]Division of Plastic Surgery,Brigham and Women’s Hospital,Harvard Medical School,Boston,MA,02215,USA

出　　处：《Bioactive Materials》2022年第12期552-568,共17页生物活性材料（英文）

基　　金：the National Natural Science Foundation of China(No.82002313,No.82072444);China Postdoctoral Science Foundation(No.2021M701333);Department of Science and Technology of Hubei Province(No.2021CFB425,No.2020BCB004);Hubei Province Key Laboratory of Oral and Maxillofacial Development and Regeneration(2021kqhm007).

摘　　要：Major traumatic tissue defects are common clinical problems often complicated by infection and local vascular dysfunction, processes which hinder the healing process. Although local application of growth factors or stem cells through various tissue engineering techniques are promising methods for the repair of tissue defects, limitations in their clinical application exist. Herein, we synthesized multifaceted nanohybrids composed of Quaternized chitosan (QCS), Graphene oxide (GO), and Polydopamine (PDA;QCS-GO-PDA). Covalent grafting of QCS and GO at a mass ratio of 5:1 (5QCS-1GO) displayed excellent biocompatibility and enhanced osteogenic ability, while addition of PDA (5QCS-1GO-PDA) reduced the level of reactive oxygen species (ROS). 5QCS-1GO-PDA was able to achieve wound tissue regeneration by reducing the inflammatory response and enhancing angiogenesis. Furthermore, Polylactic acid/hydroxyapatite (PLA/HA) composite scaffolds were printed using Selective Laser Sintering (SLS) and the hybrid nanomaterial (5QCS-1GO-PDA) was used to coat the PLA/HA scaffold (5QCS-1GO-PDA@PLA/HA) to be used for rapid bone regeneration. 5QCS-1GO-PDA not only improved angiogenesis and osteogenic differentiation, but also induced M2-type polarization of macrophages and promoted bone regeneration via the BMP2/BMPRs/Smads/Runx2 signaling pathway. The bidirectional enhanced healing ability of the multifaceted nanohybrids 5QCS-1GO-PDA provides a promising method of effectively treating tissue defects.

关 键 词：Multifunctional nanohybrids Quaternary chitosan POLYDOPAMINE ANGIOGENESIS Tissue regeneration 

分 类 号：R318[医药卫生—生物医学工程] TB3[医药卫生—基础医学]