蔗渣纤维素预处理及纤维素酶混合发酵研究  

Study on pretreatment of bagasse cellulose and mixed cellulase fermentation

作　　者：黄琦琦 黄智娴 张健[2] 付跃 何海燕[1,3,4] 覃拥灵[1,3,4] HUANG Qi-qi;HUANG Zhi-xian;ZHANG Jian;FU Yue;HE Hai-yan;QIN Yong-ling

机构地区：[1]河池学院,广西河池546300 [2]广西卫生职业技术学院,广西南宁530023 [3]河池学院微生物及植物资源开发利用广西高校重点实验室,广西河池546300 [4]河池学院农业生物技术应用研究中心,广西河池546300

出　　处：《饲料研究》2022年第14期69-73,共5页Feed Research

基　　金：广西自然科学基金(项目编号:2020GXNSFAA297218);国家自然科学基金资助项目(项目编号:31660017);河池学院2021年国家级大学生创新训练项目(项目编号:202110605012)。

摘　　要：试验旨在研究蔗渣纤维素预处理方法,优化纤维素酶系组成,提高产酶量。试验使用甘蔗渣为原料,经过微波、磷酸和蒸汽爆破预处理蔗渣纤维素。结果显示:厚垣镰孢霉HML278产纤维素酶及半纤维素酶酶活均有提高,滤纸酶酶活比未处理材料提高了1.02倍,处理后酶解葡萄糖比未处理蔗渣提高100%。米曲霉HML366可以产高转苷活性的β-葡萄糖苷酶,合成龙胆二糖,龙胆二糖可以诱导厚垣镰孢霉HML278产纤维素酶。厚垣镰孢霉HML278和米曲霉HML366菌种兼容性较好,混合发酵优化纤维素酶系组成,比厚垣镰孢霉HML278单独发酵滤纸酶活酶活提高了83.62%,β-葡萄糖苷酶比米曲霉HML366单一培养酶活提高93.09%。研究表明,米曲霉HML366和厚垣镰孢霉HML278混合培养产酶效果优于单一菌种培养产酶。The experiment was to study the pretreatment method of bagasse cellulose, optimize the composition of cellulase system and increase the amount of enzyme production. Bagasse cellulose was pretreated by microwave,phosphoric acid and steam explosion. The results showed that cellulase production and hemicellulase activity of Fusarium oxychlamycline HML278 were increased, the enzyme activity of filter paper was increased by 1.02 times than that of untreated material, and the enzymatic hydrolysis of glucose was increased by 100% compared with that of untreated bagasse. Aspergillus oryzae HML366 could produce β-glucosidase with high transferring-activity to synthesize gentiose.Gentiose could induce cellulase production from Fusarium chlamychii HML278. Fusarium oxychlamycline HML278 and Aspergillus oryzae HML366 had good compatibility, and the enzyme activity of filter paper was increased by 83.62%compared with Fusarium oxychlamycline HML278 and 93.09% compared with Aspergillus oryzae HML366. The study indicates that the mixed culture of Aspergillus oryzae HML366 and Fusarium oxychlamyclum HML278 is better than that of single strain.

关 键 词：蔗渣 纤维素酶 龙胆二糖 混合发酵 

分 类 号：S816[农业科学—饲料科学]