液相色谱串联质谱技术在病原菌鉴定中的初步应用  

Preliminary application of liquid chromatography-tandem mass spectrometry in pathogen identification

作　　者：张丽杰[1] 张璐[2] 杜燕华[3] 肖迪[4] 郑翠影 黄印启[1] 冯忠军[1] Cheng Keding ZHANG Lijie;ZHANG Lu;DU Yanhua;XIAO Di;ZHENG Cuiying;HUANG Yinqi;FENG Zhongjun;CHENG Keding(Department of Clinical Laboratory,The Third Hospital of Hebei Medical University,Shijiazhuang 050051,Hebei,China;Institute of Immunization Prevention and Planning,Henan Center for Disease Control and Prevention,Zhengzhou 450016,Henan,China;Institute of Infectious Diseases,Henan Center for Disease Control and Prevention,Zhengzhou 450016,Henan,China;State Key Laboratory of Infectious Disease Prevention and Control,Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102200,China;National Microbiology Laboratory,Public Health Agency of Canada,Winnipeg R3E 3R2,Manitoba,Canada;Department of Human Anatomy and Cell Sciences,Faculty of Medicine,University of Manitoba,Winnipeg R3E 0T5,Manitoba,Canada)

机构地区：[1]河北医科大学第三医院检验科,石家庄050051 [2]河南省疾病预防控制中心免疫预防与规划所,郑州450016 [3]河南省疾病预防控制中心传染病所,郑州450016 [4]中国疾病预防控制中心传染病预防控制所,传染病预防控制国家重点实验室,感染性疾病诊治协同创新中心,北京102200 [5]加拿大国家微生物实验室,加拿大公共卫生署,加拿大温尼伯R3E 3R2 [6]曼尼托巴大学医学院人体解剖与细胞科学系,加拿大温尼伯R3E 0T5

出　　处：《临床检验杂志》2022年第7期485-489,共5页Chinese Journal of Clinical Laboratory Science

基　　金：2020年度河北省自然科学基金精准医学联合基金培育项目(H2020206449);2016年度河北省优秀专家出国培训项目。

摘　　要：目的初步评估应用液相色谱串联质谱技术(LC-MS/MS)对病原菌进行鉴定的可行性,为该技术临床应用提供实验依据。方法以全基因基础上的蛋白质组学库作为通用细菌鉴定数据库,用LC-MS/MS技术对41株标准菌株以及临床分离的22株奈瑟菌和46株大肠埃希菌进行鉴定。结果串联质谱技术鉴定病原菌在种的水平鉴定准确率为100%。以13株标准菌株样品稀释倍数和检测获得的肽段数量进行线性分析发现,二者之间具有很好的线性关系,相关系数均在0.90以上。结论LC-MS/MS有望成为病原菌鉴定检测新方法。Objective To evaluate the feasibility of the identification for pathogens by tandem mass spectrometry(liquid chromatography-tandem mass spectrometry,LC-MS/MS)and to provides its experimental basis in clinical application.Methods A proteomic database based on the whole gene was established as a general database for bacterial identification,and 41 standard strains,22 strains of Neisseria and 46 strains of Escherichia coli isolated from clinical samples were identified by the established LC-MS/MS.Results The accuracy of the identification for pathogens at species level by tandem mass spectrometry was 100%.Based on the linear analysis of the dilution ratio of the samples from 13 standard strains and the detectable number of peptides,a good linear relationship was found between the two methods with above 0.90 of correlation coefficients.Conclusion LC-MS/MS should be promising to become a new method for identification and detection of pathogens.

关 键 词：细菌鉴定 质谱 液相色谱串联质谱 

分 类 号：R446.5[医药卫生—诊断学]