半滑舌鳎体色白化相关突变位点的鉴定  

Identification of mutation sites related to body color albinism in Cynoglossus semilaevis

作　　者：孙建华 鲍宝龙[1,2] SUN Jianhua;BAO Baolong(Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Education,Shanghai Ocean University,Shanghai 201306,China;National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学水产种质资源发掘与利用教育部重点实验室,上海201306 [2]上海海洋大学水产科学国家级实验教学示范中心,上海201306

出　　处：《上海海洋大学学报》2022年第4期849-857,共9页Journal of Shanghai Ocean University

基　　金：国家自然科学基金(31872546,31472262)。

摘　　要：对体色相关通路基因的9个错义突变位点进行测序调查,在体色黑化、体色白化和体色正常的半滑舌鳎小样本群体中发现3个突变位点与体色白化存在显著性相关,进一步通过扩大样本验证,显示B型内皮素受体第160位的C/T位点(ertB-160-C/T)与体色白化呈极显著性相关,酪氨酸酶第473的G/A位点(tyr-473-G/A)和花生四烯酸5-脂氧合酶的第1 178位的C/T位点(alox5-1178-C/T)与体色白化呈显著性相关。这3个突变位点分别位于蛋白Periplasmic_Binding_Protein_type1、Tyosinase C-terminal和Lipoxygenase结构域。qRT-PCR检测表明:ertB在白化半滑舌鳎中有眼侧正常和白化皮肤表达没有显著差异,而在无眼侧皮肤的表达显著高于有眼侧皮肤;在白化半滑舌鳎中tyr在有眼侧正常和白化皮肤的表达水平均显著高于无眼侧;白化半滑舌鳎中无眼侧alox5的表达水平均显著高于有眼侧,有眼侧白化区alox5的表达水平明显高于有眼侧正常皮肤。这些与半滑舌鳎养殖群体体色白化相关的突变位点可为今后半滑舌鳎的体色选育提供分子基础。9 missense mutation sites of body color related pathway genes were sequenced and investigated.In three small sample populations of Cynoglossus semilaevis with hyperpigmentation,pseudoalbino or normal body color,it was found that three mutation sites are significant correlation with pseudoalbino.Further verification by expanding samples showed that the C/T site at position 160 of type B endothelin receptor(ertB-160-C/T) was extremely significantly correlated with pseudoalbino.The G/A site at position 473 of tyrosinase(tyr-473-G/A) and the C/T site at position 1 178 of arachidonic acid 5-lipoxygenase(alox5-1178-C/T) were significantly correlated with pseudoalbino.The three mutation sites were located in the protein periplasmic-Binding-Protein-Type 1,tyosinase C-terminal and lipoxygenase domains.RT-qPCR assay revealed that:There was no evident difference in ertB expression in different colored areas of the skin of albino half-smooth tongue sole on the eyed side,however,the expression of ertB in the skin on the eyeless side was prominently higher than that in the skin on the eyed side,and the expression level of tyr in both pigmented and non-pigmented parts of the eyed side were remarkably higher than those of the eyeless side;The expression level of alox5 on the eyeless side was markedly higher than that on the eyed side;The alox5 expression in the albino area on the eyed side was prominently higher than that in the pigmented area on the eyed side.The mutation sites related to pseudoalbino of C.semilaevis cultured population provides a molecular basis for body color breeding of C.semilaevis in the future.

关 键 词：半滑舌鳎 体色异常 白化 突变位点 

分 类 号：S917[农业科学—水产科学]