不同饵料对克氏原螯虾仔虾养殖水质、生长、消化酶和非特异性免疫酶的影响  

Effects of different initial diets on water quality,growth,digestive enzymes and non-specific immune enzymes of Procambarus clarkii

作　　者：李晨露 李京昊 成永旭[1,2,3] 涂纹 吴乐 奚业文[4] 朱传忠 李嘉尧[1,2,3] LI Chenlu;LI Jinghao;CHENG Yongxu;TU Wen;WU Le;XI Yewen;ZHU Chuanzhong;LI Jiayao(Key Laboratory of Freshwater Aquatic Genetic Resources,Ministry of Agriculture and Rural Affairs,Shanghai Ocean University,Shanghai 201306,China;National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai 201306,China;Center for Research on Environmental Ecology and Fish Nutrion,Ministry of Agriculture and Rural Affairs,Shanghai Ocean University,Shanghai 201306,China;Aquatic Technology Promotion Station of Anhui Province,Hefei 230000,Anhui,China;Dabeinong Shenshuang Aquatic Products Technology Cmpany,Zhaoan 363500,Fujian,China)

机构地区：[1]上海海洋大学农业农村部淡水种质资源重点实验室,上海201306 [2]上海海洋大学水产科学国家级实验教学示范中心,上海201306 [3]上海海洋大学农业农村部鱼类营养与环境生态研究中心,上海201306 [4]安徽省水产技术推广总站,安徽合肥230000 [5]大北农神爽水产科技有限公司,福建诏安363500

出　　处：《上海海洋大学学报》2022年第4期883-892,共10页Journal of Shanghai Ocean University

基　　金：国家重点研发计划(2019YFD0900304);安徽省中央引导地方科技发展专项(201907d06020008);农业农村部和国家发改委产业强镇项目(发改农经2019-245);农业农村部现代农业产业技术体系项目(CARS-48)。

摘　　要：采用生物絮团、饲料和冰冻萼花臂尾轮虫(Brachionus calyciflorus) 3种饵料,对由同一亲本孵化的(0.017 9±0.000 5) g克氏原螯虾(Procambarus clarkii)仔虾进行42 d的养殖实验。比较各实验组的水体指标以及实验结束后各组仔虾的生长性能、消化酶和非特异性免疫酶活性,结果显示:在整个养殖期间,絮团组的总磷、氨氮、亚硝态氮和硝态氮浓度最低,与其他两组差异显著;3个实验组仔虾的终末体质量、增重率和特定生长率都无显著差异,絮团组的成活率显著高于饲料组,与轮虫组差异不显著;饲料组的α-淀粉酶和脂肪酶活性最高,絮团组最低,絮团组的胰蛋白酶和胃蛋白酶活性都显著高于饲料组,轮虫组的α-淀粉酶、胰蛋白酶和胃蛋白酶活性与其他两组差异不显著;絮团组的总抗氧化能力(T-AOC)和过氧化氢酶(CAT)活性最高,丙二醛(MDA)含量最少,与饲料组和轮虫组有显著差异。这3种饵料中生物絮团的养殖效果最好,对仔虾的存活、生长、消化酶和免疫抗氧化能力都有积极的作用。A 42-day study was made comparing the water indexes,growth performance,digestive enzyme and antioxidant enzyme activities of larvae red swamp crayfish(0.017 9±0.000 5) g reared feeding bioflocs,commercial diet and frozen rotifers(Brachionus calyciflorus).The results showed that:During the feeding period,the biofloc group had the lowest concentration of total phosphorus,ammonia nitrogen,nitrite nitrogen and nitrate nitrogen,significantly different from the other two groups.No significant difference was found in final weight,weight gain rate and specific growth rate of larvae crayfish among the three experimental groups while the survival rate of crayfish in the biofloc group was similar with that in the rotifer group and higher than the diet group significantly.Besides,the highest α-amylase and lipase activities were found in the diet group while the biofloc group was the lowest.The trypsin and pepsin activities of crayfish in the biofloc group were significantly higher than those in the diet group.The α-amylase,trypsin and pepsin activities of crayfish in the rotifer group were not different from the other two groups.The total antioxidant capacity(T-AOC) and catalase(CAT) activity of crayfish in the biofloc group was the highest while malondialdehyde(MDA) content was the lowest which were significantly different from the other two experimental groups.Bioflocs has more positive effects on the survival,growth performance,digestive enzymes and antioxidant capacity of larvae crayfish.

关 键 词：克氏原螯虾 生物絮团 轮虫 人工配合饲料 生长 

分 类 号：S963[农业科学—水产养殖]