腹主动脉瘤病人血清和组织中Notch1和T细胞激活分泌调节因子的表达及其意义  

Changes of serum Notch1 and RANTES expression levels in the formation of abdominal aortic aneurysm and their significance

作　　者：李光泽[1] 张济[1] 林昌俭 俞慎林[1] 包平倩[1] LI Guangze;ZHANG Ji;LIN Changjian(Department of Vascular Surgery,Leshan People's Hospital,Sichuan,Leshan 614000,China)

机构地区：[1]四川省乐山市人民医院血管外科,614000

出　　处：《临床外科杂志》2022年第7期687-690,共4页Journal of Clinical Surgery

摘　　要：目的检测腹主动脉瘤(AAA)病人血清和组织中Notch1和T细胞激活分泌调节因子(reduced upon activation mormal T expression and secreted,RANTES)的表达,分析其意义。方法2019年1月~2021年1月间我院收治AAA病人41例为AAA组,同期健康志愿者41例为健康组。通过RT-qPCR和ELISA检测血清中Notch1 mRNA和RANTES的水平。通过Western blot检测AAA组织中Notch1和RANTES蛋白表达水平。通过Pearson检测连续变量的相关性。结果AAA病人血清中Notch1 mRNA和RANTES水平分别为3.85±0.62和(58.46±5.59)μg/L健康组分别为1.04±0.23和(21.07±3.25)μg/L,两组比较差异有统计学意义(P<0.05)。AAA组织中的Notch1和RANTES蛋白表达水平分别为3.24±0.65和3.41±0.72,正常腹主动脉组织分别为1.08±0.21和1.12±0.23,两组比较差异有统计学意义(P<0.05)。AAA病人组织Notch1蛋白水平和血清Notch1 mRNA水平正相关(r=0.485,P=0.025);组织中RANTES蛋白水平与血清RAN-TES浓度正相关(r=0.506,P=0.016)。在AAA病人组织和血清中,Notch1和RANTES表达均呈正相关(r_(血清)=0.515,P=0.014;r_(组织)=0.537,P=0.008)。结论AAA病人血清和组织中的Notch1和RANTES均过表达,且二者正相关。Objective To explore the changes and significance of serum Notch1 and reduced upon activation mormal T expression and secreted(RANTES)in the formation of abdominal aortic aneurysm(AAA).Methods From January 2019 to January 2021,41 patients with AAA were selected as the AAA group.41 healthy volunteers during the same period were selected as the healthy group.The levels of Notch1 mRNA and RANTES in serum were detected by RT-qPCR and ELISA.The expression levels of Notch1 and RANTES proteins in AAA tissues were detected by Western blot.Pearson was used to detect the correlation of continuous variables.Results The levels of Notch1 mRNA and RANTES in serum of AAA patients were(3.85±0.62)and(58.46±5.59)μg/L,which were significantly higher than those of healthy individuals[(1.04±0.23),(21.07±3.25)μg/L](P<0.05).The expression levels of Notch1 and RANTES protein in AAA tissue were(3.24±0.65)and(3.41±0.72),which were significantly higher than those in normal abdominal aorta tissue[(1.08±0.21),(1.12±0.23)](P<0.05).The level of Notch1 protein in AAA tissue was positively correlated with the level of Notch1 mRNA in serum of AAA patients(r=0.485,P=0.025),and the level of RANTES protein in AAA tissue was positively correlated with the concentration of RANTES in serum(r=0.506,P=0.016).In AAA tissue and serum of AAA patients,Notch1 and RANTES showed a positive correlation(r_(serum)=0.515,P=0.014;r_(tissues)=0.537,P=0.008).Conclusion Notch1 and RANTES are over-expressed in serum and tissues of AAA patients,and the two are positively correlated.

关 键 词：腹主动脉瘤 NOTCH1 T细胞激活分泌调节因子 

分 类 号：R543.16[医药卫生—心血管疾病]