CfAtg8在调控果生刺盘孢正负菌株分化中的功能  

Function of CfAtg8 in regulating the differentiation of plus and minus strains of Colletotrichum fructicola

作　　者：孔媛媛 袁一龙 梁晓飞[1] 张荣[1] 孙广宇[1] KONG Yuanyuan;YUAN Yilong;LIANG Xiaofei;ZHANG Rong;SUN Guangyu(College of Plant Protection,Northwest A&F University,Yangling 712100,Shaanxi,China)

机构地区：[1]西北农林科技大学植物保护学院,陕西杨凌712100

出　　处：《菌物学报》2022年第8期1174-1184,共11页Mycosystema

基　　金：国家自然科学基金(32070144);国家重点研发计划(2019YFD1002002)。

摘　　要：由果生刺盘孢Colletotrichum fructicola引起的炭疽叶枯病严重危害苹果果实和叶片。刺盘孢属真菌可以产生具有形态和遗传差异的正型(+)和负型(-)菌株,但其分化的分子机制目前尚不清楚。本研究通过同源重组对Cf Atg8功能进行分析,结果显示,Cf Atg8显著影响果生刺盘孢正菌株(1104-7)菌落颜色和气生菌丝茂密程度,但不影响负菌株(1104-6);正菌株突变体不产生子囊壳,负菌株突变体子囊壳变小、数量增多;正菌株突变体与负菌株野生型1104-6杂交不形成交配线,负菌株突变体与正菌株野生型1104-7杂交形成正常交配线;此外,Cf Atg8还影响正、负菌株分生孢子萌发、附着胞形成及致病性等。这些结果表明自噬基因Cf Atg8是影响果生刺盘孢正负菌株分化、形态发育和致病的重要基因。Glomerella leaf spot caused by Colletotrichum fructicola is a severe disease on apple fruit and leaves in China.There are plus and minus strains with morphological and genetic differentiation in Colletotrichum fungi.However,the mechanisms underlying the differentiation between two types of strains remain poorly understood.The functions of CfAtg8 were analyzed based on gene deletion analysis with homologous recombination and PEG-mediated protoplast transformation.It was found that CfAtg8 significantly affected the colony color and aerial hypha density of the plus strain(1104-7),but it did not affect the minus strain(1104-6).CfAtg8 regulated the perithecial development.The plus mutant could not produce perithecia and the perithecia produced by minus mutant were more compacted and smaller in size.WhenΔ+CfAtg8/1104-6was co-cultured,mating line formation failed,while a strong mating line developed at the contact zone betweenΔ-CfAtg8/1104-7.Additionally,CfAtg8 is involved in conidial germination,appressorium formation and pathogenicity in plus and minus strains.Our results reveal that CfAtg8 is an important gene regulating genetic differentiation of plus-minus strains,morphological development and pathogenicity in Colletotrichum fructicola.

关 键 词：炭疽叶枯病 细胞自噬 致病性 非典型异宗配合 交配型 同宗配合 

分 类 号：S436.611.12[农业科学—农业昆虫与害虫防治]