miR-181a-5p介导虎杖苷体外诱导乳腺癌MDA-MB-231细胞凋亡的机制研究  

Study on the Mechanism of Apoptosis of MDA-MB-231 Cells Induced by Polydatin in Vitro Mediated by miR-181a-5p

作　　者：李釜丞 陈杏初[1] 李官成[2] LI Fu-cheng;CHEN Xin-chu;LI Guan-cheng(Department of Breast Surgery,Hunan Maternal and Child Health Hospital,Changsha Hunan 410008)

机构地区：[1]湖南省妇幼保健院乳腺外科,湖南长沙410008 [2]中南大学肿瘤研究所,湖南长沙410008

出　　处：《医学临床研究》2022年第7期1014-1017,共4页Journal of Clinical Research

摘　　要：【目的】探讨虎杖苷体外干预对乳腺癌MDA-MB-231细胞增殖及凋亡的影响。【方法】以不同浓度(0、2、4、6、8、16μmol/L)的虎杖苷处理乳腺癌MDA-MB-231细胞,MTT方法检测细胞增殖变化。乳腺癌MDA-MB-231细胞分成对照组(常规培养)、虎杖苷组(6μmol/L虎杖苷处理)、虎杖苷+Anti-NC组(转染inhibitor control,6μmol/L虎杖苷处理)、虎杖苷+Anti-miR-181a-5组(转染miR-181a-5 inhibitor,6μmol/L虎杖苷处理)。流式细胞术检测MDA-MB-231细胞凋亡情况,Western blot检测MDA-MB-231细胞Bax和c-caspase-3、caspase-3蛋白表达水平,Realtime PCR方法测定MDA-MB-231细胞miR-181a-5p表达水平。【结果】与对照组比较,2、4、6、8、16μmol/L的虎杖苷处理后的细胞吸光值显著降低(均P<0.05)。与虎杖苷+Anti-NC组比较,虎杖苷+Anti-miR-181a-5组乳腺癌细胞中miR-181a-5p水平降低,吸光值升高,细胞凋亡率降低,细胞中Bax、c-caspase-3、caspase-3蛋白水平降低(P<0.05)。与对照组比较,虎杖苷组乳腺癌细胞凋亡率升高,细胞中凋亡蛋白Bax、c-caspase-3、caspase-3水平升高,miR-181a-5p表达水平升高(P<0.05)。【结论】虎杖苷通过上调miR-181a-5p抑制乳腺癌MDA-MB-231细胞增殖并诱导细胞凋亡。【Objective】To investigate the effect of polydatin on the proliferation and apoptosis of MDA-MB-231 cells in breast cancer.【Methods】Breast cancer MDA-MB-231 cells was treated at different concentrations(0,2,4,6,8,16μmol/L)of polydatin;Cell proliferation was detected by MTT method.Breast cancer MDA-MB-231 cells were divided into control group(routine culture)and polydatin group(6μmol/L polydatin treatment),polydatin+Anti-NC group(transfection inhibitor control,6μmol/L polydatin treatment),polydatin+Anti-miR-181a-5 group(transfected with miR-181a-5 inhibitor,6μmol/L polydatin treatment).The apoptosis of MDA-MB-231 cells was detected by flow cytometry,and the expression levels of Bax,c-caspase-3 and caspase-3 proteins in MDA-MB-231 cells were detected by Western blot;The expression level of mir-181a-5p in MDA-MB-231 cells was measured by realtime PCR.【Results】Compared with the control group,2,4,6,8,16μmol/L,the absorbance value of cells treated with polydatin decreased significantly(all P<0.05).Compared with polydatin+Anti-NC group,the level of miR-181a-5p in polydatin+Anti-miR-181a-5 group decreased,the absorbance value increased,the apoptosis rate decreased,and the levels of Bax,c-caspase-3,caspase-3 protein in breast cancer cells decreased(P<0.05).Compared with the control group,the apoptosis rate of breast cancer cells in the polydatin group increased,the levels of apoptotic proteins Bax,c-caspase-3 and caspase-3 increased,and the expression level of miR-181a-5p increased(P<0.05).【Conclusion】Polydatin inhibits the proliferation and induces apoptosis of breast cancer MDA-MB-231 cells by up regulating miR-181a-5p.

关 键 词：乳腺肿瘤/病理学 微RNAS 虎杖 细胞凋亡 

分 类 号：R737.9[医药卫生—肿瘤]