食管鳞癌上皮内瘤变中雄激素受体和P53蛋白表达与异型程度的相关性研究  

Correlation between the expression of androgen receptor as well as P53 protein and the atypia degree in esophageal squamous cell carcinoma intraepithelial neoplasia

作　　者：夏秀丽 赵树山 XIA Xiuli;ZHAO Shushan(Department of Gastroenterology,Handan Central Hospital,Hebei,Handan 056001,China;不详)

机构地区：[1]河北省邯郸市中心医院消化内科,056001 [2]河北省邯郸市中心医院普外科,056001

出　　处：《河北医药》2022年第16期2431-2434,共4页Hebei Medical Journal

基　　金：河北省医学科学研究课题基金资助项目(编号:20200457)。

摘　　要：目的探讨雄激素受体(androgen receptor,AR)及P53蛋白与食管鳞癌(esophageal squamous cell carcinoma,ESCC)上皮内瘤变之间的相关性。方法选择2016年1月至2019年6月收治的120例ESCC上皮内瘤变患者标本与正常人群食管黏膜组织,其中正常、轻度、中度及重度不典型增生组织各30例。采用卵白素-生物素复合物免疫组织化学方法检测组织中AR及P53表达情况,分析AR与P53蛋白的表达与ESCC癌前病变的相关性。结果AR阳性率在正常、轻度、中度及重度不典型增生组织中依次为0,6.7%,16.7%和26.7%;P53阳性率在四组中依次为30.0%,36.7%,56.7%和73.3%。随着异型程度加重AR和P53的阳性率逐渐升高,两者表达呈正关性(r=0.995)。AR表达在正常与重度不典型增生组差异有统计学意义(χ2=7.067,P=0.008),轻度与重度不典型增生组差异有统计学意义(χ2=4.320,P=0.038),其余组间差异无统计学意义(χ2=0.517~3.491,均P>0.05)。P53表达在正常组与中、重度不典型增生组差异皆有统计学意义(χ2=4.344,P=0.037;χ2=11.279,P=0.001),轻度与重度不典型增生组差异有统计学意义(χ2=8.148,P=0.004),其余组间差异无统计学意义(P>0.05)。结论AR和P53蛋白在ESCC上皮内瘤变中呈阳性表达,并且随着异型程度加重两者的表达增加。Objective To investigate the correlation between androgen receptor(AR)as well as P53 protein and intraepithelial neoplasia of esophageal squamous cell carcinoma(ESCC).Methods A total of 120 patients with ESCC intraepithelial neoplasia who were treated in our hospital from January 2016 to June 2019 were enrolled in the study,and patients’tissue specimens were collected,moreover,the other 120 tissue specimens from esophageal mucosal tissues,including 30 cases of normal tissues,and mild,moderate and severe atypical hyperplasia tissues(30 cases each)were collected.The expression levels of AR and P53 were detected by avidin-biotin comeplex(ABC)immunohistochemical method,and the correlation between the expressions of AR,P53 protein and the precancerous lesions of ESCC was analyzed.Results The positive rates of AR in normal,mild,moderate and severe dysplasia tissues were 0,6.7%,16.7%and 26.7%,respectively.The positive rates of P53 in the four groups were 30.0%,36.7%,56.7%and 73.3%,respectively.The positive rates of AR and P53 increased gradually with the aggravation of the degree of heteromorphism,and the expressions of both were positively correlated(P<0.05).There was significant difference in AR expression between normal tissues and severe dysplasia tissues(P<0.01),and there was significant difference between mild and severe dysplasia groups(P<0.05).However there was no significant difference among the other groups(P>0.05).There were significant differences in the expression levels of P5 between the normal tissue group and the moderate as well as severe dysplasia groups(P<0.05 or P<0.01),so were between mild and severe atypical hyperplasia group(P<0.01),but,there were no significant differences among the other groups(P>0.05).Conclusion AR and P53 proteins are positively expressed in ESCC intraepithelial neoplasia,moreover,the expression levels of the two factors are increased with the aggravation of the atypia degree.

关 键 词：雄激素受体 P53 癌前病变 免疫组化 食管鳞癌 

分 类 号：R735.1[医药卫生—肿瘤]