TLR4/MyD88/NF-κB通路在真菌性角膜炎中的表达和作用  

Expression and role of TLR4/MyD88/NF-κB pathway in patients with fungal keratitis

作　　者：高峰[1] 王兵[1] 赵瑞苓[1] 王敏 张华[1] GAO Feng;WANG Bing;ZHAO Rui-ling;WANG Min;ZHANG Hua(Tengzhou Central People's Hospital,Tengzhou,Shandong 277500,China)

机构地区：[1]滕州市中心人民医院眼科,山东滕州277500

出　　处：《中华医院感染学杂志》2022年第2期237-241,共5页Chinese Journal of Nosocomiology

基　　金：山东省医药卫生科技发展计划基金资助项目(2017WS721)。

摘　　要：目的 探究Toll样受体4(TLR4)/髓样分化因子88(MyD88)/核因子κB(NF-κB)通路在真菌性角膜炎中的表达和作用。方法 选择2016年1月-2021年1月滕州市中心人民医院收治的82例(82眼)真菌性角膜炎角膜上皮组织为真菌组,另选择40例(40眼)健康角膜组织为正常组,采用免疫组化、实时荧光定量PCR(RT-PCR)和免疫印迹法检测角膜上皮中TLR4、MyD88和NF-κB蛋白表达阳性率、 mRNA及蛋白水平,酶联免疫吸附实验检测炎性细胞因子白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、γ-干扰素(IFN-γ)、α-干扰素(IFN-α)水平。结果 真菌性角膜炎角膜上皮中TLR4、MyD88和NF-κB蛋白阳性表达率、mRNA及蛋白表达水平高于正常角膜上皮(P<0.05);真菌性角膜炎角膜上皮中炎性细胞因子IL-1β、TNF-α、IFN-γ、IFN-α水平高于正常角膜上皮(P<0.05);真菌性角膜炎角膜上皮中TLR4、MyD88、NF-κB mRNA及蛋白表达水平与炎性细胞因子IL-1β、TNF-α、IFN-γ、IFN-α均呈正相关(P<0.05)。结论 TLR4、MyD88和NF-κB在真菌性角膜炎中上调表达,并与炎性细胞因子呈正相关,TLR4/MyD88/NF-κB通路的激活可能在真菌性角膜炎发病机制中发挥着重要作用。OBJECTIVE To explore the expression and role of Toll-like receptor 4(TLR4)/myeloid differentiation factor 88(MyD88)/nuclear factor κB(NF-κB) pathway in patients with fungal keratitis. METHODS A total of 82 patients(82 eyes) with fungal keratitis in corneal epithelial tissues who were treated in Tengzhou Central People′s Hospital from Jan 2016 to Jan 2021 were assigned as the fungal group, and 40 cases(40 eyes) with healthy corneal tissues were chosen as the normal group. The positive rates of expression, mRNA and proteins levels of TLR4, MyD88 and NF-κB in corneal epithelial tissues were detected by means of immunohistochemistry, real-time fluorescent quantitative PCR(RT-PCR) and Western blotting(WB), and the levels of inflammatory cytokines interleukin-1β(IL-1β), tumor necrosis factor-α(TNF-α), γ-interferon(IFN-γ) and α-interferon(IFN-α) were detected by using enzyme-linked immunosorbent assay. RESULTS The positive rates of expression, mRNA and protein levels of TLR4, MyD88 and NF-κB in corneal epithelial tissues were significantly higher in the fungal group than in the normal group(P<0.05). The levels of inflammatory cytokines IL-1β, TNF-α, IFN-γ and IFN-α in corneal epithelial tissues were significantly higher in the fungal group than in the normal group(P<0.05). The expression levels of TLR4, MyD88, NF-κB mRNA and proteins in corneal epithelial tissues of the patients with fungal keratitis were positively correlated with the inflammatory cytokines IL-1β, TNF-α, IFN-γ and IFN-α(P<0.05). CONCLUSION TLR4, MyD88 and NF-κB show up-regulated expression in the patients with fungal keratitis, which is positively correlated with the inflammatory cytokines. The activation of TLR4/MyD88/NF-κB pathway may play important role in pathogenesis of fungal keratitis.

关 键 词：真菌性角膜炎 TOLL样受体4 髓样分化因子88 核因子ΚB 

分 类 号：R772.2[医药卫生—眼科]