‘黄金芽’GST基因在不同光照条件下的表达分析  

Expression Analysis of GST Genes in ‘Huangjinya’ Under Different Light Conditions

作　　者：牛占海 王铮 宋来超 吕福堂[1] 丁兆堂 马青平 NIU Zhanhai;WANG Zheng;SONG Laichao;LYU Futang;DING Zhaotang;MA Qingping(College of Agronomy,Liaocheng University,Liaocheng,Shandong 252000;Tea Research Institute,Shandong Academy of Agricultural Sciences,Rizhao,Shandong 276800)

机构地区：[1]聊城大学农学院,山东聊城252000 [2]山东省农业科学院茶叶研究所,山东日照276800

出　　处：《北方园艺》2022年第14期31-36,共6页Northern Horticulture

基　　金：山东省自然科学基金资助项目(ZR2021QC159);山东农业良种工程资助项目(2020LZGC010);大学生创新创业训练计划资助项目(S202010447027,CXCY2021079)。

摘　　要：以茶树‘黄金芽’扦插苗为试材,对‘黄金芽’在白光、红白2:3、红白蓝3:2:1、红蓝5:1进行4种光照处理,采用实时荧光定量PCR分析4种光照处理下‘黄金芽’叶片中CsGSTs基因的表达情况,以期为深入研究光敏型茶树品种‘黄金芽’CsGSTs基因在不同光照条件下的表达提供参考依据。结果表明:与白光相比,发现处理18d后,4个茶树CsGSTs基因均是红白2:3光照条件下表达量最高,因此在红白2:3光照条件下可以诱导茶树CsGSTs的表达,相反4个CsGSTs基因在红白蓝3:2:1和红蓝5:1条件下表达量最低。说明在白光的基础上加入红光之后可以诱导CsGSTs的表达,而在白光的基础上加入蓝光之后会抑制CsGSTs的表达。Taking the cutting seedling of ‘Huangjinya’ of tea tree as the test material, the cuttings of‘Huangjinya’ were treated with white light, red-white 2∶3,red-white-blue 3∶2∶1 and red blue 5∶1.Real-time quantitative PCR was used to analyze the expression of CsGSTs genes in tender leaves of‘Huangjinya’ under four light treatments, in order to provide reference for further research on the expression of CsGSTs genes of photosensitive tea cultivar ‘Huangjinya’ under different light conditions.The results showed that compared with white light, it was found that after 18 days treatment, the expression levels of the four CsGSTs genes were the highest under the red-white 2∶3 light condition, so the expression of tea tree CsGSTs could be induced under the red and white 2∶3 light condition.On the contrary, the expression levels of the four CsGSTs genes were the lowest under the conditions of red-white-blue 3∶2∶1 and red-blue 5∶1.It shows that the expression of CsGSTs could be induced by adding red light to white light, while the expression of CsGSTs could be inhibited by adding blue to white light.

关 键 词：‘黄金芽’ CsGSTs基因 光照 表达量 

分 类 号：S571.1[农业科学—茶叶生产加工]