BLM解旋酶和TIDC在结肠癌组织中的表达及其与患者术后预后的关系研究  

Expression of BLM helicase and TIDC in colon cancer tissues and their relationship with postoperative prognosis of patients

作　　者：倪志海[1] 薛志有[1] NI Zhihai;XUE Zhiyou(Department of Anorectal Surgery,Xinhua Hospital Affiliated to Dalian University,Dalian,Liaoning 116021,P.R.China)

机构地区：[1]大连大学附属新华医院肛肠外科,辽宁大连116021

出　　处：《中国普外基础与临床杂志》2022年第8期1061-1066,共6页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的探讨Bloom综合征(Bloom syndrome,BLM)解旋酶和肿瘤浸润性树突状细胞(tumor infiltrating dendritic cells,TIDC)在结肠癌组织中的表达及其与患者术后预后之间的关系。方法回顾性选取2014年6月至2016年8月期间于大连大学附属新华医院行手术切除的168例结肠癌患者作为研究对象,获取病理科存档的手术切除的结肠癌组织以及癌旁组织标本,采用免疫组织化学染色法检测结肠癌组织以及癌旁组织中BLM解旋酶的表达及TIDC密度。采用Pearson相关分析探索BLM解旋酶表达与TIDC密度之间的相关性,采用χ2检验或秩和检验分析二者表达与结肠癌临床病理特征的关系;采用Cox比例风险回归模型分析结肠癌患者术后生存的影响因素。结果结肠癌组织中BLM解旋酶的相对表达量高于癌旁组织(1.49±0.33比1.02±0.17),TIDC密度低于癌旁组织[(9.53±2.36)%比(12.36±2.37)%],其差异均有统计学意义(P<0.05)。Pearson相关性分析结果显示,BLM解旋酶的表达与TIDC密度呈负相关关系(r=–0.588,P<0.05)。BLM解旋酶的表达和TIDC密度与肿瘤分化程度、临床分期及淋巴结转移具有相关性(P<0.05),即BLM解旋酶高表达和TIDC低密度者的肿瘤分化程度低、临床分级晚、淋巴结转移比例高。168例患者均获随访,随访时间16~60个月,中位随访时间45个月,随访期间有63例患者死亡(37.5%)。log-rank分析结果显示,BLM解旋酶低表达组的5年累积生存率高于高表达组,TIDC低密度组的5年累积生存率低于高密度组,其差异均有统计学意义(P<0.05)。Cox比例风险回归模型分析结果显示,BLM解旋酶高表达、TIDC低密度、肿瘤分化程度低、分期晚和伴淋巴结转移是影响结肠癌患者术后生存的危险因素(P<0.05)。结论结肠癌组织中BLM解旋酶表达和TIDC密度的异常,与肿瘤分化程度和淋巴结转移相关,是影响结肠癌患者远期生存的危险因素。Objective To investigate the expression of Bloom syndrome(BLM)helicase and tumor infiltrating dendritic cells(TIDC)in colon cancer tissues and their relationship with the prognosis of patients after surgery.Methods One hundred and sixty-eight patients with colon cancer who underwent surgical resection in our hospital from June 2014 to August 2016 were selected as the research objects.The specimens of surgically resected colon cancer tissues and adjacent tissues archived by the pathology department were obtained,and the expression of BLM helicase and TIDC density were detected by immunohistochemistry.Pearson was used to analyze the correlation between BLM helicase expression and TIDC density,and the relationship between the expression of BLM helicase and TIDC density and the clinicopathological features of colon cancer was analyzed by usingχ2 test or Wilcoxon rank test.The influencing factors of postoperative survival of patients with colon cancer were analyzed by Cox proportional hazards regression model.Results The relative expression of BLM helicase in colon cancer tissues was higher than that in adjacent tissues(1.49±0.33 vs.1.02±0.17),while the TIDC density was lower than that in adjacent tissues[(9.53±2.36)%vs.(12.36±2.37)%],the differences were statistically significant(P<0.05).Pearson correlation analysis showed that there was a negative correlation between the expression of BLM helicase and TIDC density(r=–0.588,P<0.05).The expression of BLM helicase and TIDC density were correlated with tumor differentiation,clinical stage and lymph node metastasis(P<0.05).That is,those with high expression of BLM helicase and low density of TIDC had low degree of tumor differentiation,late clinical grade,and higher ratio of lymph node metastasis.Sixty-three cases died(37.5%)during the follow-up period(16–60 months,medium follow up 45 months).Log-rank analysis showed that the 5-year cumulative survival rate of the BLM helicase-low expression group was higher than that of the high expression group,and that of the

关 键 词：结肠癌 Bloom综合征解旋酶 肿瘤浸润性树突状细胞 预后标志物 

分 类 号：R735.35[医药卫生—肿瘤]