贵紫麦SLC25A4基因参与花青素合成的功能初步研究  

Preliminary Analysis on the Functions of SLC25A4 Gene in Anthocyanin Synthesis of Guizi Wheat(Triticum aestivum)

作　　者：李鲁华 熊富敏 安畅 彭亚姝 任明见[1,2] 徐如宏[1,2] LI Lu-Hua;XIONG Fu-Min;AN Chang;PENG Ya-Shu;REN Ming-Jian;XU Ru-Hong(College of Agriculture,Guizhou University,Guiyang 550025,China;Guizhou Sub-Center of National Wheat Improvement Center,Guiyang 550025,China)

机构地区：[1]贵州大学农学院,贵阳550025 [2]国家小麦改良中心贵州大学分中心,贵阳550025

出　　处：《农业生物技术学报》2022年第7期1237-1246,共10页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(32160456);贵州省科技计划项目(黔科合基础[2019]1073;[2020]1Z018);贵州省科技支撑项目([2021]一般272)。

摘　　要：溶质转运家族25(solute carrier family 25,SLC25)成员参与花青素合成的调控过程。其家族成员SLC25A4是腺嘌呤转移酶1(adenine nucleotide translocase 1,ANT1),参与植物生长发育和植物对逆境胁迫响应等生物学过程。为了丰富小麦(Triticum aestivum)SLC25A4的研究内容,探讨其在调控花青素合成方面的生物学功能。本研究深入挖掘不同发育时期’贵紫麦1号’籽粒转录组数据中SLC25A4的表达情况并进行qPCR验证,发现花后25天和花后35天(day post-anthesis,DPA)TaSLC25A4-7A、TaSLC25A4-7B和TaSLC25A4-7D1基因的表达量显著高于10 DPA(P<0.05),TaSLC25A4-7A基因表达水平的变化趋势与花青素积累量的变化趋势一致。本研究进一步获得了TaSLC25A4-7A过表达烟草(Nicotiana tabacum)转基因株系,发现转基因株系中花青素合成相关基因NtDFR,Nt4CL,NtCHI和NtF3H的表达量显著高于野生型(P<0.05),表明TaSLC25A4-7A参与调控花青素合成的生物学过程。用羰基氰化物间氯苯腙(carbonyl cyanide m-chlorophenylhydrazone,CCCP)处理过表达转基因株系和野生型发现,过表达转基因株系对CCCP处理更敏感。本研究结果表明,TaSLC25A4基因(特别是TaSLC25A4-7A)参与调控花青素的合成,为深入探讨TaSLC25A4基因在小麦花青素积累方面的生物学功能提供参考。The solute carrier family 25(SLC25)members participate in regulation of anthocyanin biosynthesis.The SLC25 family member SLC25A4 is adenine nucleotide translocase 1(ANT1),which plays important roles in growth and development,and response to stresses of plant.To enrich the research of SLC25A4 in wheat(Triticum aestivum)and explore biological functions of SLC25A4 in the regulation of anthocyanin biosynthesis,the expression of SLC25A4 was analyzed based on transcriptome sequence results at different developmental stages of’Guizi 1’wheat grains and was further verified by qPCR.The results demonstrated that the expression of TaSLC25A4-7A,TaSLC25A4-7B and TaSLC25A4-7D1 genes was significantly higher at 25 days post anthesis(DPA)and 35 DPA than that at 10 DPA(P<0.05),particularly qPCR analysis showed that the dynamic change of TaSLC25A4-7A gene expression was consistent with that of anthocyanin accumulation.Furthermore,TaSLC25A4-7A gene overexpression transgenic tobacco(Nicotiana tabacum)lines were obtained,qPCR results showed that the expression level of anthocyanin synthesis related genes(NtDFR,Nt4CL,NtCHI and NtF3H)was significantly higher in transgenic lines than that in wild type(P<0.05),which demonstrated that TaSLC25A4-7A involved in the biological process of regulating anthocyanin synthesis.The results of carbonyl cyanide m-chlorophenylhydrazone(CCCP)treatment to transgenic lines and wild type demonstrated that the TaSLC25A4-7A overexpression transgenic lines were more sensitive to CCCP.The above results demonstrated that TaSLC25A4 gene(especially TaSLC25A4-7A)is involved in anthocyanin synthesis,which provides reference for further exploring the biological function of the TaSLC25A4 gene in anthocyanin accumulation and stresses response of wheat.

关 键 词：溶质转运家族25 SLC25A4 转录组 花青素 

分 类 号：S512.1[农业科学—作物学]