不同GR24浓度对藻技术净化沼液沼气效果的影响  

Effects of GR24 with Different Concentrations on Purification of Biogas and Biogas Slurry by Algae Technology

作　　者：李先科 许兵[2] 赵永军[3] 徐劼[3] 韦静 LI Xianke;XU Bing;ZHAO Yongjun;XU Jie;WEI Jing(Liaocheng Kehui Municipal Engineering Design Co.,Ltd.,Liaocheng 252000,China;School of Municipal and Environmental Engineering,Shandong Jianzhu University,Jinan 250101,China;College of Biological,Chemical Science and Engineering,Jiaxing University,Jiaxing 314001,China;College of Advanced Materials Engineering,Jiaxing Nanhu University,Jiaxing 314001,China)

机构地区：[1]聊城市科慧市政工程设计有限公司,山东聊城252000 [2]山东建筑大学市政与环境工程学院,山东济南250101 [3]嘉兴学院生物与化学工程学院,浙江嘉兴314001 [4]嘉兴南湖学院新材料工程学院,浙江嘉兴314001

出　　处：《环境科学与技术》2022年第7期20-29,共10页Environmental Science & Technology

基　　金：国家自然科学基金项目(31971514,31670511);浙江省自然科学基金项目(LY16C030003,LQ17E080013);嘉兴南湖学院重点项目(N41472001-43)。

摘　　要：该文以3种处理系统(小球藻-灵芝菌-内生细菌、小球藻-灵芝菌以及小球藻单培养)为研究对象,在独脚金内酯(GR24)4种浓度诱导下同步处理沼液和沼气,研究GR24对小球藻的生物量、光合作用的影响及沼液的净化和沼气的升级效果。随着GR24浓度的增加,微藻系统中共生体代谢和微藻的光合作用增强,共生体快速生长,净化性能增强。此外,通过GR24的诱导增加了微藻胞内碳酸酐酶活性,从而提高共培养体系的CO_(2)去除性能。小球藻-灵芝菌-内生细菌共培养系统显著提高了光合速率、生物量、沼液营养物质去除效果以及沼气转化率。在最佳条件下(GR24浓度为10^(-9)mol/L),沼液中COD、TN和TP的平均去除率分别为(84.45%±8.13%)、(80.29%±7.86%)和(84.96%±8.23%),沼气中CO_(2)的平均去除率为(65.37%±6.14%)。Three systems(Chlorella vulgaris-Ganoderma lucidum-endophytic bacteria;Chlorella vulgaris-Ganoderma lucidum and single-cultured Chlorella vulgaris)were set up for a bench-scale experiment,with which biogas slurry and biogas were simultaneously treated.A kind of phyto-hormone named GR24(prepared in solutions with four different concentrations)was used to induce the treatment,and the experiment research focused on the effects of GR24 on Chlorella vulgaris in terms of the biomass and the photosynthesis,and on how the purification of biogas slurry and biogas was upgraded by the induction of GR24 with four different concentrations.As the solution concentration of GR24 added into the systems increased,so did the symbiote metabolism and photosynthesis of microalgae induced by GR24,the symbiote grew rapidly,which further enhanced the purification performance of the systems.In addition,the intracellular carbonic anhydrase activity of microalgae was enhanced by the induction of GR24,thus improving the CO_(2) removal performance of the co-culture systems.In summary,the co-culture system of Chlorella vulgaris-Ganoderma lucidum-endophytic bacteria significantly improved the photosynthetic rate,biomass,nutrient removal efficiency and biogas conversion rate.Under the optimal condition(GR24 concentration of 10^(-9)mol/L),the average removal rates of COD,TN and TP in biogas slurry were(84.45%±8.13%),(80.29%±7.86%)and(84.96%±8.23%),respectively,and the average removal rate of CO_(2) in biogas was(65.37%±6.14%).

关 键 词：小球藻 共生系统 独角金内酯 光合性能 去除性能 

分 类 号：X17[环境科学与工程—环境科学]