秦川牛FABP4、FASN和TCAP基因多态性位点与生长和胴体性状的关联性分析  

Association Analysis Between Polymorphisms in FABP4, FASN and TCAP Genes and Growth and Carcass Traits in Qinchuan Cattle(Bos taurus)

作　　者：迟志娇 澈力木格 成功[2] 苏雅 成立新[4] 刘剑锋[5] 曹贵方[6] 包斯琴 昝林森[2] 佟彬 CHI Zhi-Jiao;Chelmeg;CHENG Gong;SU Ya;CHENG Li-Xin;LIU Jian-Feng;CAO Gui-Fang;BAO Si-Qin;ZAN Lin-Sen;TONG Bin(State Key Laboratory of Reproductive Regulation&Breeding of Grassland Livestock/School of Life Sciences,Inner Mongolia University,Hohhot 010071,China;National Beef Cattle Improvement Center/College of Animal Science and Technology,Northwest A&F University,Yangling 712100,China;Inner Mongolia Agricultural and Animal Husbandry-Tech Extension Center,Hohhot 010011,China;Animal Husbandry Institute,Inner Mongolia Academy of Agricultural&Animal Husbandry,Hohhot 010031,China;Key Laboratory of Animal Genetics,Breeding and Reproduction/National Engineering Laboratory for Animal Breeding,Ministry of Agriculture/College of Animal Science and Technology,China Agricultural University,Beijing 100193,China;Department of Veterinary,Inner Mongolia Agricultural University,Hohhot 010018,China)

机构地区：[1]内蒙古大学省部共建草原家畜生殖调控与繁育国家重点实验室/生命科学学院,呼和浩特010071 [2]西北农林科技大学国家肉牛改良中心/动物科技学院,杨凌712100 [3]内蒙古自治区农牧业技术推广中心,呼和浩特010011 [4]内蒙古农牧业科学院畜牧研究所,呼和浩特010031 [5]中国农业大学农业部畜禽遗传育种与繁殖重点实验室/农业部畜禽育种国家工程实验室/动物科学技术学院,北京100193 [6]内蒙古农业大学兽医学院,呼和浩特010018

出　　处：《农业生物技术学报》2022年第8期1488-1498,共11页Journal of Agricultural Biotechnology

基　　金：国家重点研发计划(2018YFD0501700);内蒙古自治区重大专项(2020ZD0007);内蒙古自治区应用技术研究与开发资金计划(20180260);省部共建草原家畜生殖调控与繁育国家重点实验室(内蒙古大学)自主课题(2021ZZ0204);陕西省重点研发计划(2019NY-098);人社部高层次留学人才回国资助项目(人社厅函[2018]190号);内蒙古自治区“草原英才”工程(CYYC6068);内蒙古大学高层次人才引进科研启动金(21400-5165112)。

摘　　要：肉牛(Bos taurus)的生长速度和胴体品质在肉牛产业中具有重要的经济价值。脂肪酸结合蛋白4(fat acid binding proteins 4,FABP4)和脂肪酸合酶(fatty acid synthase,FASN)在脂肪酸的合成过程中具有关键作用,是影响肉牛脂肪沉积和脂肪酸组成的关键功能基因。肌联蛋白帽(titin-cap,TCAP)作为一种肌丝蛋白在肌原纤维的组装过程中发挥着重要作用。TCAP基因g.346G>A位点、FASN基因g.17924G>A位点和FABP4基因g.3691G>A位点与肉牛的生长、胴体和肉质性状高度相关。为探究上述3个位点对秦川牛生长和胴体性状的影响及其在不同肉牛品种中的多态性,本研究利用基质辅助激光解析电离飞行时间质谱(matrix-assisted laser desorption ionization time of flight mass spectrometry,MassARRAY)技术对350头秦川牛的以上3个基因的3个位点进行了基因分型和遗传多态性分析,并分析了其与秦川牛生长和胴体性状的关联性。结果表明,FASN基因g.17924G>A位点、FABP4基因g.3691G>A位点及TCAP基因g.346G>A位点在本研究所选的秦川牛群体中均符合哈迪-温伯格平衡。在350头秦川牛群体中,FASN基因g.17924G>A位点与秦川牛尻长与腰角宽显著相关(P<0.05),有益等位基因(G)频率与韩国牛和安格斯牛差异极显著(P<0.01)。FABP4基因g.3691G>A位点与秦川牛腰高、腰角宽和胸围显著相关(P<0.05),与体重、体斜长、尻长和胸深极显著相关(P<0.01),有益等位基因(G)频率与韩国牛差异极显著(P<0.01)。TCAP基因g.346G>A位点与秦川牛体高与腰高极显著相关,与体重、体斜长、坐骨端宽和肌内脂肪含量显著相关(P<0.05),有益等位基因(G)频率与韩国牛差异极显著(P<0.01)。综上所述,TCAP基因g.346G>A位点、FASN基因g.17924G>A位点和FABP4基因g.3691G>A位点与秦川牛的生长和胴体性状显著相关。本研究结果可为秦川牛分子标记辅助育种提供有效分子遗传标记,为秦川牛的遗传改良提供参考依据。The growth rate and carcass quality of beef cattle(Bos taurus) have important economic value in beef cattle industry. Fat acid binding proteins 4 gene(FABP4) and fatty acid synthase gene(FASN) play important role in the process of fatty acid synthesis and fat deposition in beef cattle. Titin-cap(TCAP) gene is known to be a kind of muscle silk protein and plays important role in the assembly of myofibrils. There are a g. 3691G>A SNP in FABP4 gene, a g. 17924G>A SNP in FASN gene and a g. 346G>A SNP in TCAP gene,which were associated with growth and carcass traits in beef cattle. The aims of this study were to investigate the genetic diversity of g. 3691G>A, g. 17924G>A and g. 346G>A SNPs in Qinchuan cattle population, and confirm their association with growth and carcass traits in Qinchuan cattle. These 3 SNPs of 3 genes in 350samples of Qinchuan cattle were genotyped by MassARRAY technology, and then the analyses of genetic diversity and association with growth and carcass traits in Qinchuan cattle were performed. The results showed that the genotype frequency of g.3691G>A SNP in FABP4 gene, g.17924G>A SNP in FASN gene and g.346G>A SNP in TCAP gene were consistent with Hardy-Weinberg equilibrium(P<0.05) in Qinchuan cattle population. Association analysis results showed that g. 17924G>A SNP of FASN gene in Qinchuan cattle population was significantly associated with rump length and hip width(P<0.05), as well as beneficial alleles(G) frequency was significantly different from that of Korean and Angus cattle breeds(P<0.01). Association analysis results showed that g.3691G>A SNP of FABP4 gene in Qinchuan cattle population was significantly associated with hip height, hip width and chest circumference(P<0.05), as well as body weight, body length,rump length and chest depth(P<0.01), beneficial alleles(G) frequency was significantly different from that of Korean cattle(P<0.01). Association analysis results showed that g. 346G>A SNP of TCAP gene in Qinchuan cattle population was significantly associated with withers heigh

关 键 词：秦川牛 脂肪酸结合蛋白4基因(FABP4) 脂肪酸合酶基因(FASN) 肌联蛋白帽基因(TCAP) 生长性状 胴体性状 

分 类 号：S8[农业科学—畜牧兽医]