A geranylgeranyl pyrophosphate synthase gene, Ib GGPS, increases carotenoid contents in transgenic sweetpotato  

作　　者：LI Rui-jie ZHAI Hong HE Shao-zhen ZHANG Huan ZHAO Ning LIU Qing-chang 

机构地区：[1]Key Laboratory of Sweetpotato Biology and Biotechnology,Ministry of Agriculture and Rural Affairs/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization,Ministry of Education/College of Agronomy&Biotechnology,China Agricultural University,Beijing 100193,P.R.China

出　　处：《Journal of Integrative Agriculture》2022年第9期2538-2546,共9页农业科学学报（英文版）

基　　金：supported by the National Key Research and Development Program of China(2019YFD1001302 and 2019YFD1001300);the China Agriculture Research System of MOF and MARA(CARS-10-Sweetpotato)。

摘　　要：Geranylgeranyl pyrophosphate synthase(GGPS) plays an important role in the biosynthesis of carotenoids. In a previous study, the IbGGPS gene was isolated from a sweetpotato, Ipomoea batatas(L.) Lam., line Nongdafu 14 with high carotenoid contents, but its role and underlying mechanisms in carotenoid biosynthesis in sweetpotato were not investigated. In the present study, the IbGGPS gene was introduced into a sweetpotato cv. Lizixiang and the contents of β-carotene, β-cryptoxanthin, zeaxanthin and lutein were significantly increased in the storage roots of the IbGGPSoverexpressing sweetpotato plants. Further analysis showed that IbGGPS gene overexpression systematically upregulated the genes involved in the glycolytic, 2-C-methyl-D-erythritol-4-phosphate(MEP) and carotenoid pathways,which increased the carotenoid contents in the transgenic plants. These results indicate that the IbGGPS gene has the potential for use in improving the carotenoid contents in sweetpotato and other plants.

关 键 词：SWEETPOTATO Ipomoea batatas(L.)Lam. IbGGPS OVEREXPRESSION CAROTENOID 

分 类 号：S531[农业科学—作物学]