Association of 2-methoxyestradiol levels with the occurrence and development of endometrial cancer in humans  

作　　者：Huanhuan Zhao Junyu Li Yan Liu Li Li 

机构地区：[1]Department of Obstetrics and Gynecology,The Forth Hospital,Hebei Medical University,Shijiazhuang 050011,China [2]Department of Pharmacy,Hebei Medical University,Shijiazhuang 050017,China

出　　处：《Oncology and Translational Medicine》2022年第4期191-195,共5页肿瘤学与转化医学（英文版）

基　　金：Supported by the Hebei Province Medical Science Research Key Project(No.20210276).

摘　　要：Objective The aim of the study was to determine the association of urinary levels of estradiol(E_(2))and 2-methoxyestradiol(2-MeOE_(2))with the occurrence and development of endometrial cancer.Methods In this case-control study,24-h urine specimens were collected from 28 postmenopausal patients with endometrial cancer and 28 postmenopausal healthy female controls.The concentration of 2-MeOE_(2) was determined using liquid chromatography-mass spectrometry with hollow fiber liquid-phase microextraction.The concentration of E_(2) was determined using an enzyme-linked immunosorbent assay.Results Estrogen levels were different between the patients with endometrial cancer and controls.The relative quantity of E_(2) in the case group was higher than that in the control group(P<0.05),whereas that of 2-MeOE_(2) was lower in the case group than that in the control group(P<0.05).The ratio of E_(2)-to-2-MeOE_(2) in the case group was significantly higher than that in the control group(P<0.05).Conclusion The results of this study indicate an imbalance of estrogen metabolites in endometrial carcinogenesis.Reduced 2-MeOE_(2) levels and elevated E_(2)-to-2-MeOE_(2) ratio may be used as potential biomarkers for the risk assessment of estrogen-induced endometrial cancer.

关 键 词：endometrial cancer 2-methoxyestradiol(2-MeOE_(2)) estradiol(E_(2)) URINE high-performance liquid chromatography-mass spectrometry(HPLC-MS) 

分 类 号：R737.33[医药卫生—肿瘤]