circ_0001982靶向miR-578对乳腺癌细胞自噬、增殖及细胞周期影响的机制研究  

Study on the effect of circ_0001982/miR-578 on autophagy,proliferation and cell cycle of breast cancer cells

作　　者：杜全顺 李少华[1] 马成云 贾振飞[1] 王现兵[1] 王嘉毅[1] DU Quan-shun;LI Shao-hua;MA Cheng-yun;JIA Zhen-fei;WANG Xian-bing;WANG Jia-yi(Department of General Surgery,Anyang People's Hospital of Henan Provine,Anyang 455100,China)

机构地区：[1]河南省安阳市人民医院普外科,河南安阳455100

出　　处：《中国现代普通外科进展》2022年第8期589-593,共5页Chinese Journal of Current Advances in General Surgery

摘　　要：目的:探讨环状RNA0001982(circ_0001982)对乳腺癌细胞自噬、增殖和细胞周期的影响及分子机制。方法:RT-qPCR检测乳腺癌患者癌组织和癌旁组织circ_0001982和miR-578表达水平。将乳腺癌细胞MCF-7分为NC组、si-NC组、si-circ_0001982组、pc DNA-circ_0001982组、miR-578组和pc DNA-circ_0001982+miR-578组。RT-qPCR检测circ_0001982和miR-578表达水平;四甲基偶氮唑盐比色法(MTT)和流式细胞术检测细胞增殖和周期;Western blot法检测Cyclin D1、p21、LC3-Ⅰ、LC3-Ⅱ蛋白表达;双荧光素酶报告实验验证circ_0001982和miR-578的关系。结果:乳腺癌组织和MCF-7细胞中circ_0001982表达水平升高,miR-578表达水平降低(P<0.05)。敲低MCF-7细胞circ_0001982可降低细胞活性和G2/M期细胞比例,减少Cyclin D1、LC3-Ⅰ蛋白表达,升高G0/G1期细胞比例,促进p21、LC3-Ⅱ蛋白表达。circ_0001982具有靶向调控miR-578作用。结论:敲低circ_0001982可能通过上调miR-578抑制乳腺癌MCF-7细胞自噬、增殖及阻滞细胞周期。Objective:To explore the effect of circ_0001982 on breast cancer cell autophagy,proliferation and cell cycle and its molecular mechanism.Methods:Detection of expression levels of circ_0001982 and miR-578 in cancer tissues and adjacent tissues of breast cancer patients by real-time quantitative PCR(RT-qPCR),and divide breast cancer cell MCF-7 into NC group,si-NC group,si-circ_0001982 group,pc DNA-circ_0001982 group,miR-578 group,pc DNA-circ_0001982+miR-578 group.Real-time fluorescent quantitative PCR(RT-qPCR)was used to detect the expression of circ_0001982 and miR-578;the tetramethylazolium salt colorimetric method(MTT)was used to detect the cell proliferation activity rate;the Western blot method was used to detect Cyclin D1、p21、LC3-Ⅰ、LC3-Ⅱprotein expression;flow cytometry detects the cell cycle;the dual luciferase report experiment verifies the targeting relationship between circ_0001982 and miR-578.Results:The expression level of circ_0001982 in breast cancer tissues and cells was increased,and the expression level of miR-578 was decreased(P<0.05).Knockdown of circ_0001982 reduced cell viability,Cyclin D1,LC3-I expression levels,and G2/M phase cell ratio,while p21,LC3-II expression levels,and G0/G1 phase cell ratio increased.circ_0001982 targets and regulates miR-578;overexpression of circ_0001982 and miR-578 at the same time reduces cell viability,Cyclin D1,LC3-I expression levels,G2/M cell ratio,p21,LC3-II expression level,G0/G1 cell ratio increased(P<0.05).Conclusions:Knockdown of circ_0001982 may inhibit the autophagy,proliferation and cell cycle arrest of breast cancer MCF-7 cells by up-regulating miR-578.

关 键 词：乳腺肿瘤 circ_0001982 miR-578 自噬 增殖 细胞周期 

分 类 号：R737.9[医药卫生—肿瘤]