CRISPR/Cas9全基因组筛选技术在疾病治疗中的应用及在眼科应用中的展望  

Application of CRISPR/Cas9 Genome-Wide Screening Technology in Disease Treatment and the Prospect of Its Application in Ophthalmology

作　　者：周佳木 张贝贝 王艳歌 胡旭萌 葛可可 李盼 宋宗明 ZHOU Jiamu;ZHANG Beibei;WANG Yange;HU Xumeng;GE Keke;LI Pan;SONG Zongming(Department of Ophthalmology,People’s Hospital of Zhengzhou University/Henan Provincial People’s Hospital/Henan Eye Hospital/Henan Eye Institution,Zhengzhou 450003,China)

机构地区：[1]郑州大学人民医院/河南省人民医院/河南省立眼科医院/河南省眼科研究所眼科,河南郑州450003

出　　处：《河南医学研究》2022年第16期3068-3072,共5页Henan Medical Research

基　　金：国家自然科学基金(82101162);河南省眼科研究所基础研究专项(20JCZD001)。

摘　　要：CRISPR/Cas9全基因组筛选技术可在全基因组范围内快速筛选具有特定生物学功能的DNA片段,不仅能反映疾病发生、发展过程中目的基因表达的变化,还能为药物进一步开发与应用提供重要依据。目前,该技术已经在肿瘤治疗、耐药性研究及病毒感染等方面得到广泛的应用。在眼科疾病研究中,CRISPR/Cas9基因编辑技术能够对目的基因进行快速精准的编辑,但目前大量编码或非编码序列的功能及疾病耐药机制仍有待进一步探究,全基因组筛选技术的应用将为其发展开辟新的道路。The DNA fragments with specific biological functions can be rapidly screened by CRISPR/Cas9 genome-wide screening technology in the whole genome which not only reflect the changes in the expression of the target gene in the occurrence of diseases,but also provide the important basis for further drug development and application.At present,the technology has been widely used in tumor treatment,drug-resistance research and virus infection.The CRISPR/Cas9 technology can perform rapid and precise gene editing of target genes in the research of ophthalmic diseases,but the functions of a large number of coding or non-coding sequences remain to be elucidated.The application of genome-wide screening technology will open up new avenues for its development.

关 键 词：CRISPR 全基因组筛选 眼科 疾病治疗 

分 类 号：R77[医药卫生—眼科]