巴马汀对BRAF/KRAS突变非小细胞肺癌细胞增殖凋亡和顺铂敏感性的影响  

Effects of Palmatine on Proliferation Apoptosis and Cisplatin Sensitivity in BRAF/KRAS Mutant Non-Small Cell Lung Cancer Cells

作　　者：黄亮[1] 黄旭 张冠男 HUANG Liang(The Affiliated Hospital of Chengde Medical University,Hebei Chengde 067000,China)

机构地区：[1]承德医学院附属医院肿瘤科,河北承德067000 [2]北京市大兴区中西医结合医院普胸外科,北京大兴区100076

出　　处：《河北医学》2022年第8期1247-1251,共5页Hebei Medicine

基　　金：河北省医学科学研究重点课题计划,(编号:20120163)。

摘　　要：目的:探讨巴马汀对BRAF/KRAS突变非小细胞肺癌细胞(NSCLC)增殖、凋亡和顺铂敏感性的影响。方法:采用BRAF/KRAS双突变NSCLC细胞系A549为研究对象,分别利用噻唑蓝法和流式细胞术检测巴马汀对A549细胞增殖活力、细胞凋亡和顺铂敏感性的影响。Western blotting检测Bcl-2和PARP蛋白表达。结果:与对照组比较,1μmoL/L、5μmoL/L和10μmoL/L组细胞的增殖活力、Bcl-2和PARP蛋白表达均显著降低,细胞凋亡率显著升高(P<0.05)。与对照组比较,巴马汀组和顺铂组细胞的增殖活力、Bcl-2和PARP蛋白表达均显著降低,细胞凋亡率显著升高(P<0.05);与顺铂组比较,顺铂+巴马汀组细胞的增殖活力、Bcl-2和PARP蛋白表达进一步降低,细胞凋亡率进一步升高(P<0.05)。结论:巴马汀可抑制BRAF/KRAS突变NSCLC细胞增殖,诱导细胞凋亡,并增强顺铂的化疗敏感性。Objective:To investigate the protective effect and mechanism of Palmatine on proliferation,apoptosis and cisplatin sensitivity in BRAF/KRAS mutant non-small cell lung cancer cells(NSCLC).Methods:The BRAF/KRAS mutant NSCLC cell line A549 was used as the research subject,and the effects of Palmatine on cell proliferation,apoptosis and cisplatin sensitivity were detected by thiazolyl blue method and flow cytometry,respectively.Western blotting was used to detect the expression of Bcl-2 and PARP protein.Results:Compared with the control group,the proliferation viability,Bcl-2 and PARP protein expression were significantly lower and the apoptosis rate was significantly higher in the 1μmol/L,5μmol/L and 10μmol/L groups(P<0.05).Compared with the control group,the proliferation viability,Bcl-2 and PARP protein expression were significantly reduced and the apoptosis rate was significantly increased in the palmatine and cisplatin groups(P<0.05);compared with the cisplatin group,the proliferation viability,Bcl-2 and PARP protein expression were further reduced and the apoptosis rate was further increased in the cisplatin+palmatine group(P<0.05).Conclusion:Palmatine can inhibit the proliferation of BRAF/KRAS mutant NSCLC cells,induce apoptosis,and enhance the sensitivity of cisplatin.

关 键 词：巴马汀 BRAF突变 KRAS突变 非小细胞肺癌 

分 类 号：R734.2[医药卫生—肿瘤]