黄化茶树黄金菊的叶绿体结构透射电镜观察  

Transmission Electron Microscopic Study of Chloroplast Structure of Yellow Tea Cultivar Huangjinju

作　　者：江新凤 李琛 曹挥华 王治会 江和源[2] 董春旺[2] 倪德江[3] 童忠飞 JIANG Xin-feng;LI Chen;CAO Hui-hua;WANG Zhi-hui;JIANG He-yuan;DONG Chun-wang;NI De-jiang;TONG Zhong-fei(Jiangxi Research Institute of Cash Crops/Key Laboratory of Tea Quality and Safety Control in Jiangxi Province,Nanchang 330043,China;Tea Research Institute,Chinese Academy of Agricultural Sciences,Hangzhou 310008,China;College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]江西省经济作物研究所/江西省茶叶质量与安全控制重点实验室,江西南昌330043 [2]中国农业科学院茶叶研究所,浙江杭州310008 [3]华中农业大学园艺林学学院,湖北武汉430070

出　　处：《江西农业学报》2022年第6期52-57,共6页Acta Agriculturae Jiangxi

基　　金：江西省现代农业产业技术体系(JXARS-02、JXARS-15);江西省茶叶质量与安全控制重点实验室(20192BCD40007);江西省科技合作专项重点项目(20212BDH80011、20212BDH8025);江西省重点研发计划一般项目(20212BBF63009)。

摘　　要：利用透射电镜技术研究了江西特有黄化茶树资源黄金菊与正常茶树品种宁州2号的第2叶、第4叶的亚细胞叶绿体结构差异。结果发现遮荫的黄金菊与宁州2号在亚细胞结构和分布规律方面基本一致,但是叶绿体结构差异明显。黄金菊第2叶叶绿体数量偏少,基粒片层堆叠明显少于宁州2号;且黄金菊第4叶基粒片层堆叠多于第2叶,但仍少于宁州2号。叶绿体片层堆叠情况与其含量测定的结果基本一致。同时发现,遮荫处理后的黄金菊叶色变绿,叶绿体片层堆叠也明显增多。研究结果表明,黄金菊黄化可能与叶绿体片层合成受阻有关,该结果为深入了解黄金菊黄化的机理提供了数据支撑。The subcellular chloroplast structure of the second and fourth leaves of yellow tea cultivar Huangjinju and normal green tea cultivar Ningzhou 2 were studied by transmission electron microscope. It was found that there were no significant differences in subcellular structure and distribution. However, there were significant differences in chloroplast structure.Compared to Ningzhou 2, smaller chloroplasts and less grana lamellae were found in Huangjinju. The chloroplast lamellae numbers were obviously less than those of Ningzhou 2. The grana lamellae of the fourth leaf were more than those of the second leaf, but still less than those of Ningzhou 2. The chloroplast lamellae numbers were consistent with the chlorophyll contents determined. Furthermore, the leaves of Huangjinju turned green, and the chloroplast lamellae numbers increased under shading treatment. The results indicated that the yellow of Huangjinju was correlated with the biosynthesis of chloroplast lamellae, which can provide data support for further understanding its yellowing mechanism in Huangjinju.

关 键 词：茶树 黄化茶树 黄金菊 叶绿体 透射电镜 

分 类 号：S571.1[农业科学—茶叶生产加工]