牦牛血清对H9c2细胞增殖及衰老的影响  

Effect of yak serum on proliferation and senescence of H9c2 cells

作　　者：白玉婷[1] 格日力[2] 王立生[3] 苏晓灵[1] BAI Yuting;GE Rili;WANG Lisheng;SU Xiaoling(Department of Cardiovascular Medicine,Qinghai Provincial People’s Hospital,Xining,Qinghai 810001,China;Research Center for High Altitude Medicine,Medical College,Qinghai University,Xining,Qinghai 810001,China;Department of Molecular Diagnosis and Regenerative Medicine,Medical Research Center,Affiliated Hospital of Qingdao University,Qingdao,Shandong 266000,China)

机构地区：[1]青海省人民医院心血管内科,西宁810001 [2]青海大学医学院高原医学研究中心,西宁810001 [3]青岛大学附属医院医学研究中心分子诊断与再生医学科,山东青岛266000

出　　处：《重庆医学》2022年第16期2835-2838,共4页Chongqing medicine

基　　金：青海省心血管疾病临床医学研究中心建设项目(2019-SF-L1)。

摘　　要：目的研究在其他培养条件相同的情况下不同类型新生牛血清对H9c2细胞体外培养的影响,为更加合理选择H9c2细胞生长适应的血清类型提供参考依据。方法采用牦牛血清、Hyclone血清、四季青血清3种牛血清对H9c2细胞进行培养,通过细胞计数法和CCK8法测定细胞生长的细胞数量及活力,并在镜下观察细胞形态,以比较3种血清细胞培养的效果,采用q-PCR测定p21、p16、p38、p53表达情况以了解细胞衰老情况。结果牦牛血清培养细胞的细胞数在第2~6天均显著高于四季青血清,在第2天显著高于Hyclone血清,差异均有统计学意义(P<0.05);其他时间点比较,差异均无统计学意义(P>0.05);Hyclone血清培养细胞的细胞数在第3~6天均显著高于四季青血清(P<0.05)。牦牛血清培养细胞的细胞倍增数在第24、48、72、96、120小时均显著高于Hyclone血清和四季青血清,差异均有统计学意义(P<0.05),Hyclone血清培养细胞的细胞倍增数在第24小时显著高于四季青血清,差异均有统计学意义(P<0.05),其余时间点比较,差异均无统计学意义(P>0.05);此外,与牦牛血清和Hyclone血清比较,四季青血清H9c2细胞p21、p53表达最高。结论牦牛血清的细胞培养效果优于Hyclone血清和四季青血清,H9c2细胞可能更适应于在牦牛血清中生长。Objective To investigate the influence of different types of newborn calf serum on H9 c2 cells in vitro culture under the same other culture conditions to provide the reference for more reasonably select suitable serum of H9 c2 cell growth.Methods The three kinds of yak serum,hyclone serum and Sijiqing serum were adopted to culture the H9 c2 cells.The cells number and the cell vitality were detected by the cell count method and CCK8 method.The cellular morphology was observed by microscope to compare the cell culture effects.The expression levels of p21,p16,p38 and p53 were measured by Q-PCR in order to understand the cell senescence situation.Results The cells number cultured by yak serum on 2,6 d was significantly higher than that by Sijiqing serum(P<0.05),its cells number on 2 d was significantly higher than that in the hyclone group(P<0.05),and the difference at other time points had no statistical significance(P>0.05).The cells number cultured by hyclone serum on 3-6 d was significantly higher than that in the Sijiqing serum(P<0.05);the doublings number of the cells cultured by yak serum at 24,48,72,96,120 h was significantly higher than that in the hyclone serum and Sijiqing serum,and the difference was statistically significant(P<0.05),the doublings number of the cells cultured by hyclone serum at 24 h was significantly higher than that in the Sijiqing serum,and the difference was statistically significant(P<0.05),and the difference at other time points had no statistical significance(P>0.05);in addition,compared with the yak serum and hyclone serum,the expression level of p21 and p53 in the Sijiqing serum H9 c2 cells was the highest.Conclusion The cell culture effect of yak serum is better than that of hyclone serum and Sijiqing serum.H9 c2 cells may be more adapt to grow in the yak serum.

关 键 词：牦牛血清 H9C2细胞 细胞增殖 细胞衰老 

分 类 号：Q253[生物学—细胞生物学]