siRNA介导MAGE-3沉默抗老年大鼠胃癌活性作用及相关机制  

Effect and mechanism of siRNA mediated MAGE-3 silencing on gastric cancer activity in aged rats

作　　者：杨梅 皮国涛 高欢 柳英辉[1] 陈文习[1] YANG Mei;PI Guo-Tao;GAO Huan(Gastroenterology Department,Ezhou Central Hospital,Ezhou 436000,Hubei,China)

机构地区：[1]鄂州市中心医院消化内科,湖北鄂州436000

出　　处：《中国老年学杂志》2022年第17期4249-4253,共5页Chinese Journal of Gerontology

基　　金：国家自然科学基金委员会资助项目(81602117)。

摘　　要：目的探讨siRNA介导黑色素瘤抗原基因(MAGE)-3沉默抗老年大鼠胃癌活性的作用及相关机制。方法选取30只清洁级SD雄性老年大鼠,建立胃癌模型,成功27只,随机分为模型组、对照组和MAGE-3沉默组各9只。建立靶向MAGE-3基因寡核苷酸,观察胃癌肿瘤生长状况、病理学、胃癌细胞增殖情况,检测端粒酶催化亚单位(hTERT)、信号转导与激活因子(STAT)3、p53、血管内皮生长因子(VEGF)蛋白表达。结果与模型组相比,对照组肿瘤质量、肿瘤体积升高,MAGE-3沉默组肿瘤质量、肿瘤体积下降,差异有统计学意义(P<0.05)。与对照组相比,MAGE-3沉默组肿瘤质量、肿瘤体积降低,抑瘤率增加,差异有统计学意义(P<0.05)。与模型组相比,对照组胃癌细胞增殖指数增加,MAGE-3沉默组胃癌细胞增殖指数下降,差异有统计学意义(P<0.05)。与对照组相比,MAGE-3沉默组胃癌细胞增殖指数下降,差异有统计学意义(P<0.05)。与模型组相比,对照组hTERT、STAT3、p53、VEGF蛋白表达升高,MAGE-3沉默组hTERT、STAT3、p53、VEGF蛋白表达降低,差异有统计学意义(P<0.05)。与对照组相比,hTERT、STAT3、p53、VEGF蛋白表达降低,差异有统计学意义(P<0.05)。结论siRNA介导MAGE-3沉默能抑制老年大鼠胃癌肿瘤生长,通过调控hTERT、STAT3、p53、VEGF蛋白表达,抑制胃癌活性。Objective To study the role and mechanism of siRNA mediated melanoma antigen gene(MAGE)-3 silencing on gastric cancer in aged rats.Methods Thirty old male SD rats of clean grade were selected to establish gastric cancer model.27 rats were randomly divided into model group,control group and MAGE-3 silencing group with 9 rats in each group.MAGE-3 gene oligonucleotide was established to observe the growth of gastric cancer,rat pathology,gastric cancer cell proliferation,to detect telomerase catalytic subunit(hTERT),signal transducer and activator(STAT)3,p53,vascular endothelial growth factor(VEGF)protein expression.Results Compared with the model group,the tumor mass and volume in the control group were increased,the tumor mass and volume of the MAGE-3 silencing group were decreased,the difference was statistically significant(P<0.05).Compared with the control group,the tumor mass and volume of MAGE-3 silencing group were decreased,the tumor inhibition rate was increased,and the difference was statistically significant(P<0.05).Compared with the model group,the proliferation index of gastric cancer cells in the control group was increased,while that in the MAGE-3 silencing group was decreased,and the difference was statistically significant(P<0.05).Compared with the control group,the proliferation index of gastric cancer cells in MAGE-3 silencing group decreased,and the difference was statistically significant(P<0.05).Compared with the model group,the expressions of hTERT,STAT3,p53 and VEGF in the control group were increased,while the protein expression of hTERT,STAT3,p53 and VEGF in the MAGE-3 silencing group decreased,and the differences were statistically significant(P<0.05).Compared with the control group,hTERT,STAT3,p53,VEGF protein expression decreased,the differences were statistically significant(P<0.05).Conclusions siRNA mediated MAGE-3 silencing could inhibit the growth of gastric cancer in aged rats,and inhibit the activity of gastric cancer by regulating the expression of hTERT,STAT3,p53 and VEGF.

关 键 词：SIRNA 黑色素瘤抗原基因-3 胃癌 

分 类 号：R573[医药卫生—消化系统]