体外培养的兔下颌骨髁突对机械压力的反应和变化  被引量:2

Reaction and changes of rabbit mandibular condyle cultured in vitro to mechanical compression

在线阅读下载全文

作  者:李辉[1] 黄林剑 谢千阳[1] 杨驰[1] LI Hui;HUANG Lin-jian;XIE Qian-yang;YANG Chi(Department of Oral Surgery,Shanghai Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine,College of Stomatology,Shanghai Jiao Tong University,National Center for Stomatology,National Clinical Research Center for Oral Diseases,Shanghai Key Laboratory of Stomatology.Shanghai 200011;Department of Oral and Maxillofacial Surgery,Second Affiliated Hospital Zhejiang University School of Medicine.Hangzhou 310009,Zhejiang Province,China)

机构地区:[1]上海交通大学医学院附属第九人民医院口腔外科,上海交通大学口腔医学院,国家口腔医学中心,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海200011 [2]浙江大学医学院附属第二医院口腔颌面外科,浙江杭州310009

出  处:《中国口腔颌面外科杂志》2022年第4期340-346,共7页China Journal of Oral and Maxillofacial Surgery

基  金:上海交通大学医工(理)交叉基金(YG2019QNB08)。

摘  要:目的:探索体外培养的下颌骨髁突组织块对机械压力的反应和变化。方法:兔下颌骨髁突组织块分别在0(对照)、15和75 kPa机械压力下体外培养3 d,观察髁突的大体形态、组织切片,进行micro-CT扫描,分别采用RT-PCR和Western免疫印迹检测Ⅱ型胶原蛋白(COL2)、X型胶原蛋白(COL10)、SOX9和MMP13的mRNA及蛋白表达。采用SPSS 16.0软件包对数据进行统计学分析。结果:15 kPa组,髁突关节面光滑,软骨呈白色,关节面中心受压后变平,但软骨仍完整。75 kPa组,髁突关节面变粗糙,颜色变黄、暗淡,关节面中央受压变平的面积更大,中心区域软骨破裂。镜下可见,15 kPa组的软骨层变薄,软骨细胞排列比对照组更紧密。75 kPa组中,整个软骨层被破坏并与中心区域的软骨下骨分离。COL2和SOX9的表达在15 kPa压力下显著增加(P<0.01),在75 kPa压力下显著下降。COL10和MMP13的表达在75 kPa压力下显著增加(P<0.01)。Micro-CT扫描结果显示,压力组的骨密度(BMD)、骨体积/总体积比值(BV/TV)和骨小梁厚度(Tb.Th)显著增加(P<0.01)。结论:体外培养的髁突组织块在15 kPa机械压力下保持完整性和功能,并发生适应性改建。75 kPa的压力对软骨产生破坏,软骨层受损、破裂并从软骨下骨处剥离,可能进一步导致骨关节炎的发生。PURPOSE:To investigate the changes of rabbit mandibular condyle under mechanical compression in vitro.METHODS:Mandibular condylar explants were cultured in vitro,and subjected to static pressures of 0(control),15 and75 kPa for 3 days,respectively.The gross morphology,histology and expression of collagen II(COL2),collagen X(COL10),SOX9 and MMP13 were examined,and micro-CT was performed.Statistical analysis was carried out using SPSS 16.0 software package.RESULTS:In 15 kPa group,the condyle was still intact and white with flat remodeled articular surface.In75 kPa group,the remodeled articular surface was rough,and flat area was larger than that in 15 kPa group.Condyles be-came dulling and yellow with cracked cartilage in the center of articular surface.The morphological structure of the condylar cartilage and chondrocytes were more compacted than those in the control group.In the 75 kPa group,the whole layer of cartilage was damaged and detached from the subchondral bone in the central area.The expression of COL2 and SOX9 increased significantly under the pressure of 15 kPa(P<0.01),and decreased significantly at 75 kPa.The expression of COL10 and MMP13 increased significantly under the pressure of 75 kPa(P<0.01).Micro-CT showed a significant increase in bone mineral density(BMD),bone volume/total volume(BV/TV)and trabecular thickness(Tb.Th)in the pressurized groups(P<0.01).CONCLUSIONS:The integrity and function of condylar explants were maintained under the mechanical loading of 15 kPa,and adaptive remodeling was observed.Degenerative changes in cartilage were observed at 75 kPa.The pressure of 75 kPa exceeded the junction bond of cartilage and subchondral bone,and resulted in detachment of cartilage from subchondral bone which may lead to osteoarthritis.

关 键 词:颞下颌关节 下颌骨髁突 机械压力 改建  

分 类 号:R782.6[医药卫生—口腔医学]

 

参考文献:

正在载入数据...

 

二级参考文献:

正在载入数据...

 

耦合文献:

正在载入数据...

 

引证文献:

正在载入数据...

 

二级引证文献:

正在载入数据...

 

同被引文献:

正在载入数据...

 

相关期刊文献:

正在载入数据...

相关的主题
相关的作者对象
相关的机构对象