Cariporide对癫痫模型大鼠心肌细胞凋亡的影响  

Effect of cariporide on cardiomyocyte apoptosis in epileptic rats

作　　者：马鹏飞 彭爽 谢懂君 吴旭玲 郑乾 冯占辉 MA Pengfei;PENG Shuang;XIE Dongjun(Department of Neurology,the Affiliated Hospital of Guizhou Medical Universiy,Guiyang(550004),Guizhou,China)

机构地区：[1]贵州医科大学附属医院神经内科,贵州贵阳550004

出　　处：《癫痫与神经电生理学杂志》2022年第4期193-196,202,共5页Journal of Epileptology and Electroneurophysiology(China)

基　　金：国家自然科学基金委员会资助项目(81860248);贵州医科大学国家自然科学基金培育项目(19NSP051);贵州省卫健委科学技术基金项目(gzwjkj2020-2-005);中国抗癫痫协会癫痫科研基金-UCB基金(2018016)。

摘　　要：目的 探讨Cariporide对癫痫模型大鼠心肌细胞凋亡的影响。方法 选取SD雄性大鼠45只,氯化锂—匹罗卡品构建癫痫模型作为模型组(n=30),腹腔注射生理盐水作为对照组(n=15),采用Cariporide干预癫痫模型鼠作为干预组(n=15)。第60天麻醉处死大鼠后取心肌组织,应用蛋白印迹和RT-PCR检测钠氢交换体1(NHE-1)的表达,采用流式细胞检测大鼠心肌细胞凋亡情况。结果 制作模型过程中7只大鼠死亡,死亡率为23%。第60天蛋白印迹结果显示干预组大鼠较模型组心肌细胞NHE-1蛋白表达降低(P<0.05)。RT-PCR结果显示干预组大鼠较模型组心肌细胞NHE-1 mRNA表达降低(P<0.05)。流式细胞检测显示干预组大鼠较模型组心肌细胞凋亡率明显降低(P<0.05)。结论 Cariporide可通过下调NHE-1来改善癫痫模型大鼠心肌细胞凋亡情况。Objective To investigate the effect of Cariporide on cardiomyocyte apoptosis in rat epilepsy model. Methods Forty-five male SD rats were selected. The rats were injected with lithium chloride pilocarpine as model group(n=30), and the rats were intraperitoneally injected normal saline as control group(n=15). Cariporide was used to intervene rats with epilepsy as an intervention group(n=15). On the 60day, the rats were anesthetized and sacrificed, and myocardial tissues were collected. Western blot and RT-PCR were used to detect the expression of sodium-hydrogen exchanger isoform-1(NHE-1). Flow cytometry was used to detect the apoptosis of rat cardiomyocytes. Results Seven rats died in the process of establishing the model, and the mortality rate was 23%. On the 60 th day, Western blot showed that NHE-1 protein expression in cardiomyocyte of intervention group was lower than that of the model group(P<0.05). RT-PCR results showed that NHE-1 mRNA expression in the cardiomyocyte was lower in intervention group than that in model group(P<0.05). Flow cytometry showed that the apoptosis rate of cardiomyocyte in intervention group was significantly lower than that in model group(P<0.05). Conclusion Cariporide can improve cardiomyocyte apoptosis by downregulating NHE-1 in rat epilepsy model.

关 键 词：心肌 钠氢交换体1(NHE-1) 细胞凋亡 癫痫大鼠模型 CARIPORIDE 

分 类 号：R742.1[医药卫生—神经病学与精神病学]