高效液相法测定饲料中黄曲霉素B1的不确定度分析  

Evaluation of uncertainty in determination of aflatoxin B1 in feed by high-performance liquid chromatography

作　　者：邓田方 吴玉刚 胡蝶 徐焕宇 郑会祥 DENG Tianfang;WU Yugang;HU Die;XU Huanyu;ZHENG Huixiang(Guangdong Yuehai Feeds Group Co.Ltd.,Zhanjiang 524000,China)

机构地区：[1]广东粤海饲料集团股份有限公司,广东湛江524000

出　　处：《养殖与饲料》2022年第9期53-57,共5页Animals Breeding and Feed

基　　金：发酵中草药制备复合型促生长饲料添加剂专利成果转化与产业化(2020A04006);2019年度省科技专项资金(“大专项+任务清单”)竞争性分配项目:抗病型高级虾苗开口饵料专利成果转化与应用推广(2019A04004)。

摘　　要：[目的]评定亲和柱净化-高效液相色谱法测定饲料中黄曲霉素B1(AFB1)的测定方法的不确定度。[方法]参考国家标准《饲料中黄曲霉毒素B_(1)、B_(2)、G_(1)、G_(2)的测定免疫亲和柱净化-高效液相色谱法》(GB/T 30955—2014),利用高效液相法测定饲料中AFB_(1)的含量,分析并评估检测过程中样品前处理、标准曲线绘制、方法重复性引入的不确定度的方法。[结果]饲料中AFB_(1)的含量为(2.1±0.2)μg/kg时,其合成标准不确定度和扩展不确定度分别为0.047、0.2μg/kg,=2。[结论]不确定度主要来自标准曲线和重复性试验的标准偏差引入的不确定度,其中主要是标准曲线及标准溶液的配制贡献最大。[Objectives]To evaluate the uncertainty of the determination method of aflatoxins B1(AFB1)in feed,high performance liquid chromatography was used to determine the content of AFB1 in feed.[Methods]According to the national standard“Determination of aflatoxins B_(1),B_(2),G_(1),G_(2) in feed,pu-rification of immunoaffinity column-High-performance liquid chromatography”(GB/T 30955-2014).The uncertainty was analyzed and evaluated from sample pretreatment,standard curve drawing and method re-peatability.[Results]When the content of AFB_(1) was(2.1±0.2)μg/kg in feed,the synthetic standard un-certainty and the expanded uncertainty of results were 0.047μg/kg and 0.2μg/kg,=2,respectively.[Conclusions]The uncertainty mainly comes from the uncertainty introduced by the standard curve and the standard deviation introduced by the repeatability test.Meantime,the uncertainty introduced by the standard curve and the standard solution has the greatest impact on the results.

关 键 词：饲料 亲和柱净化-高效液相色谱法 黄曲霉毒素B1 不确定度 

分 类 号：O657.72[理学—分析化学] S816.17[理学—化学]