一株高效微生物絮凝菌的筛选与鉴定  

Screening and Identification of A High Efficiency Bioflocculant

作　　者：吴丽红[1] 邵楠 韩红玉 孙瑞婷 冯邱思 杜荣杰 Wu Lihong;Shao Nan;Han Hongyu;Sun Ruiting;Feng Qiusi;Du Rongjie(Liaoning Institute of Science and Technology,Benxi 117002,P.R.China)

机构地区：[1]辽宁科技学院,辽宁·本溪117002

出　　处：《东北林业大学学报》2022年第8期122-124,128,共4页Journal of Northeast Forestry University

基　　金：辽宁科技学院大学生创新创业训练计划项目(202211430033)。

摘　　要：以某污水处理厂二沉池剩余活性污泥为菌种来源进行絮凝菌的分离筛选,经富集培养、分离、初筛、复筛、优选等,优选出一株高效的絮凝菌,对高岭土悬浮液具有很好的絮凝沉降效果。对菌株进行形态观察、生理生化试验及16S rDNA的序列测定和同源性分析结果表明,优选出的菌株为贝莱斯芽孢杆菌(Bacillus velezensis)。菌株最佳生长时间是分批培养后12~48 h,培养48 h左右的稳定期菌种可作为菌种絮凝活性研究对象。A sewage treatment flocculant producing bacteria was isolated from the residual activated sludge of a secondary sedimentation tank in a sewage treatment plant. After enrichment culture, isolation, primary screening, re-screening and optimization, a highly efficient flocculant was selected, it has good flocculation and sedimentation effect on kaolin suspension. The results of morphological observation, physiological and biochemical tests, 16 S rDNA sequence determination and homology analysis showed that the strain was Bacillus velezensis. The optimal growth time of the strain was 12-48 h after batch cultivation, and the stable stage strain cultivated for could be used as the research object of flocculation activity.

关 键 词：絮凝菌 微生物絮凝菌 贝莱斯芽孢杆菌 

分 类 号：Q939.9[生物学—微生物学] X172[环境科学与工程—环境科学]