硫酸-蒽酮法用于灵芝孢子粉中粗多糖的检测研究  

Determination of Crude Polysaccharides in Ganoderma Lucidum Spore Powder by Sulfuric Acid Anthrone Method

作　　者：柴俪洪 杨恒 张艳华 张丽娟 杨仝 Chai Lihong;Yang Heng;Zhang Yanhua;Zhang Lijuan;Yang Tong(Tianjin Kangting Bioengineering Group Co.,Ltd.,Tianjin 300385)

机构地区：[1]天津市康婷生物工程集团有限公司,天津300385

出　　处：《山西化工》2022年第5期43-44,49,共3页Shanxi Chemical Industry

摘　　要：采用硫酸-蒽酮法探究提取过程和测定过程的较优条件。研究结果表明,优化条件:提取过程中水浴时间90min,浸提频次20min/次;在测定过程中乙醇浓度80%,80%硫酸浓度。改进后的硫酸蒽酮法测定灵芝孢子粉RSD为1.7%,回收率在92.4%~97.1%。The sulfuric acid anthrone method was used to explore the better conditions of the extraction process and determination process.The results showed that the optimal conditions were as follows:the water bath time was 90 min,and the extraction frequency was 20 min/time.In the determination process,the volume fraction of ethanol is 80%,and the concentration of sulfuric acid is 80%.The RSD of ganoderma lucidum spore powder determined by the improved anthrone sulfate method is 1.7%,and the recovery is between 92.4%and 97.1%.

关 键 词：硫酸-蒽酮法 粗多糖 优化条件 

分 类 号：TQ460.7[化学工程—制药化工]