三磷酸腺苷结合盒式亚家族C成员8在脑胶质瘤组织中的表达及预后预测价值  

Expression of ABCC8 mRNA in gliomas and its application value as a prognostic indicator

作　　者：周开甲[1] 刘彦伟[2] 赵征 张鸣[1] 刘伯伟[1] Zhou Kaijia;Liu Yanwei;Zhao Zheng;Zhang Ming;Liu Bowei(Neuro-Oncology Surgery Department of Fujian Medical University Cancer Hospital,Fujian Cancer Hospital,Fuzhou 350014,China;Beijing Neurosurgical Institute,Capital Medical University,Beijing 100070,China)

机构地区：[1]福建医科大学附属肿瘤医院福建省肿瘤医院神经肿瘤外科,福州350014 [2]首都医科大学北京神经外科研究所,北京100070

出　　处：《中华肿瘤杂志》2022年第8期836-841,共6页Chinese Journal of Oncology

基　　金：福建省卫健委科技计划中青年骨干人才培养项目(2021GGA044)。

摘　　要：目的探讨脑胶质瘤组织中三磷酸腺苷结合盒式亚家族C成员8(ABCC8)mRNA的表达及其作为预测脑胶质瘤预后评估指标的应用价值。方法收集中国脑胶质瘤基因组图谱(CGGA)数据库中304例脑胶质瘤患者(研究组)的性别、年龄、组织病理类型、WHO分级、IDH突变、1p/19q缺失、生存时间和ABCC8 mRNA表达水平等数据,采用单因素及多因素Cox回归分析明确脑胶质瘤患者的预后影响因素,并通过癌症基因组图谱(TCGA)数据库的506例脑胶质瘤患者、分子脑肿瘤数据库(REMBRANDT)的271例脑胶质瘤患者进行验证。结果研究组304例脑胶质瘤组织中ABCC8 mRNA表达量为15.93±1.23。ABCC8 mRNA表达量与患者的年龄、组织病理类型、WHO分级、异柠檬酸脱氢酶(IDH)突变状态、1p/19q缺失状态、WHO分子分级有关(均P<0.01)。ABCC8 mRNA高表达组患者的中位生存时间为85.60个月,明显长于低表达组(14.07个月,P<0.001)。单因素Cox回归分析显示,年龄、组织病理类型、WHO分级、IDH突变状态、1p/19q缺失状态、术后放疗、术后化疗及ABCC8 mRNA表达与患者总生存时间有关(均P<0.05)。多因素Cox回归分析显示,WHO分级(HR=3.117,95%CI:1.970~4.930)、1p/19q缺失状态(HR=0.285,95%CI:0.155~0.526)、术后化疗(HR=0.680,95%CI:0.488~0.948)及ABCC8 mRNA表达(HR=0.690,95%CI:0.491~0.971)为脑胶质瘤患者生存时间的独立影响因素。在TCGA数据集中,ABCC8 mRNA高表达组患者的中位生存时间为133.70个月,长于低表达组患者(19.60个月,P<0.001)。在REMBRANDT数据集中,ABCC8 mRNA高表达组患者中位生存时间为37.93月,长于低表达组患者(15.83个月,P<0.001)。结论脑胶质瘤组织中ABCC8 mRNA的表达水平与肿瘤恶性程度有关,恶性程度越高,表达越低。ABCC8 mRNA的表达水平是脑胶质瘤患者预后的独立影响因素,ABCC8 mRNA高表达者预后好于低表达者。Objective To investigate the mRNA expression of adenosine triphosphate binding cassette subfamily C member 8(ABCC8)in gliomas and its application value as an index for predicting the prognosis of gliomas.Methods The data of sex,age,histopathological type,WHO grade,isocitrate dehydrogenase(IDH)mutation,1p/19q deletion,survival time and ABCC8 mRNA expression of 304 patients with gliomas(study group)in the Chinese glioma Genome Atlas(CGGA)database were collected.The prognostic factors of patients with gliomas were determined by univariate and multivariate Cox regression analysis.This result was verified by the data of 506 glioma patients in TCGA database and 271 glioma patients in REMBRANDT database.Results The expression of ABCC8 mRNA in 304 cases of gliomas in the study group was 15.93±1.23.ABCC8 mRNA expression was correlated with age,histopathological type,WHO grade,IDH mutation status,1p/19q deletion status and WHO molecular grade(P<0.01).The median survival time of patients in ABCC8 mRNA high expression group was 85.60 months,which was significantly longer than 14.07 months in low expression group(P<0.001).Univariate Cox regression analysis showed that age,histopathological type,WHO grade,IDH mutation status,1p/19q deletion status,postoperative radiotherapy,postoperative chemotherapy and ABCC8 mRNA expression were related to the overall survival time of patients(P<0.05).Multivariate Cox regression analysis showed that WHO grade(HR=3.117,95%CI:1.970-4.930),1p/19q deletion status(HR=0.285,95%CI:0.155-0.526),postoperative chemotherapy(HR=0.680,95%CI:0.488-0.948)and ABCC8 mRNA expression(HR=0.690,95%CI:0.491-0.971)were independent factors for the overall survival of patients.In the TCGA dataset,the median survival time of patients with high ABCC8 mRNA expression group was 133.70 months,which was longer than 19.60 months of patients with low ABCC8 mRNA expression group(P<0.001).In Rembrandt dataset,the median survival time of patients with high ABCC8 mRNA expression group was 37.93 months,which was longer than 15.

关 键 词：脑胶质瘤 三磷酸腺苷结合盒式亚家族C成员8 分子分型 预后 

分 类 号：R739.41[医药卫生—肿瘤]