The sex determination gene doublesex regulates expression and secretion of the basement membrane protein Collagen Ⅳ  

作　　者：Qionglin Peng Jiangtao Chen Rong Wang Huan Zhu Caihong Han Xiaoxiao Ji Yufeng Pan 

机构地区：[1]The Key Laboratory of Developmental Genes and Human Disease,School of Life Science and Technology,Southeast University,Nanjing,Jiangsu 210096,China [2]Co-innovation Center of Neuroregeneration,Nantong University,Nantong,Jiangsu 226019,China

出　　处：《Journal of Genetics and Genomics》2022年第7期636-644,共9页遗传学报（英文版）

基　　金：supported by grants from National Key R&D Program of China (2019YFA0802400);the National Natural Science Foundation of China (31970943 and 31700905);the Jiangsu Innovation and Entrepreneurship Team Program

摘　　要：The highly conserved doublesex(dsx) and doublesex/mab-3 related(Dmrt) genes control sexually dimorphic traits across animals. The dsx gene encodes sex-specific transcription factors, Dsx^(M) in males and Dsx^(F) in females, which function differentially and often oppositely to establish sexual dimorphism. Here, we report that mutations in dsx, or overexpression of dsx, result in abnormal distribution of the basement membrane(BM) protein Collagen Ⅳ in the fat body. We find that Dsx isoforms regulate the expression of Collagen Ⅳ in the fat body and its secretion into the BM of other tissues. We identify the procollagen lysyl hydroxylase(dPlod) gene, which is involved in the biosynthesis of Collagen Ⅳ, as a direct target of Dsx. We further show that Dsx regulates Collagen Ⅳ through d Plod-dependent and independent pathways. These findings reveal how Dsx isoforms function in the secretory fat body to regulate Collagen Ⅳ and remotely establish sexual dimorphism.

关 键 词：DROSOPHILA Sexual dimorphism DOUBLESEX Dmrt genes CollagenⅣ dPlod Fat body 

分 类 号：Q75[生物学—分子生物学]