癫痫持续状态大鼠海马周细胞活化及胶质瘢痕形成的实验研究  

The activation of hippocampal pericytes and glial scar formation in rats with status epilepticus

作　　者：丁丹蕊 雷洁 冉蕗希 王华[1] Ding Danrui;Lei Jie;Ran Luxi;Wang Hua(Department of Pediatric Neurology,Shengjing Hospital of China Medical University,Shenyang 110004,China;Department of Neurology,Chengdu Second People′s Hospital,Chengdu 610017,China)

机构地区：[1]中国医科大学附属盛京医院小儿神经内科,沈阳110004 [2]成都市第二人民医院神经内科,610017

出　　处：《国际儿科学杂志》2022年第7期488-492,F0003,共6页International Journal of Pediatrics

基　　金：国家重点研发计划(2016YFC1306203)。

摘　　要：目的探讨癫痫持续状态(status epilepticus,SE)后血管周围反应性周细胞活化,以及周细胞活化与星形胶质细胞增殖和功能的关系。方法将80只大鼠随机分为对照组16只、模型组64只(造模后1 d、3 d、7 d、28 d组各16只),应用腹腔注射氯化锂-匹罗卡品诱导SE模型,行脑组织切片苏木精-伊红染色,观察基本病理改变。应用免疫组化技术、Western blot检测神经元-胶质抗原2(neuron-glial antigen 2,NG2)表达,并应用免疫荧光技术对NG2与胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)进行双染,观察其相互关系。结果模型组神经元排列紊乱,失去带状结构,神经元出现变性、坏死,可观察到神经元的细胞核模糊,细胞质边集,有较多胶质细胞增生。与对照组相比,模型组NG2在SE后呈现动态高表达(P<0.05);周细胞数量显著升高,在7 d时达到峰值,Western blot的结果与免疫组化结果一致(P<0.05);并且周细胞在周围区域诱导聚集胶质细胞,参与胶质细胞信号传导。结论周细胞可以诱导胶质细胞聚集,在SE脑损伤后以胶质瘢痕的形式参与修复。Objective To explore the perivascular activation of reactive pericytes after status epilepticus(SE),and the relationship between pericytes and glial cells in proliferation and function.Methods Eighty rats were randomly divided into control group(n=16)and model group(n=64,16 for each group in SE1d,SE3d,SE7d,SE28d).The SE model was induced by intraperitoneal injection of lithium chloride and pilocarpine,and hematoxylin-eosin staining was performed on brain tissue sections to observe basic pathological changes.Use immunohistochemistry and Western blot to detect(neuron-glial antigen 2,NG2)expression,and use immunofluorescence technology to double stain NG2 and(glial fibrillary acidic protein,GFAP)to observe their relationship.Results In the model group,the neurons were arranged disorderly,losing the ribbon structure,and the neurons appeared degeneration and necrosis.It was observed that the nuclei of the neurons were blurred,and the cytoplasm was agglomerated.There were more glial cells proliferation.Compared with the control group,it was found in model group that NG2 showed a dynamic high expression after SE(P<0.05).The number of pericytes increased significantly,reaching a peak at 7d,and the results of Western blot were consistent with the results of histochemistry(P<0.05).The aggregation of glial cells were induced in the surrounding area,and pericytes participated in the signal transduction of glial cells.Conclusion Pericytes can induce the aggregation of glial cells and participate in the repairment in the form of glial scars after SE brain injury.

关 键 词：大鼠 周细胞 胶质细胞 胶质瘢痕 癫痫持续状态 

分 类 号：R742.1[医药卫生—神经病学与精神病学]