抗宫炎软胶囊的质量标志物分析  

Q-marker analysis of Kanggongyan soft capsule

作　　者：袁敏艳 张敏 张硕 曹思源 季嘉城 王鹏娇 张荣平 高秀丽 YUAN Minyan;ZHANG Min;ZHANG Shuo;CAO Siyuan;JI Jiacheng;WANG Pengjiao;ZHANG Rongping;GAO Xiuli(State Key Laboratory of Function and Application of Medicinal Plants/School of Pharmacy,Guizhou Medical University,Guiyang 550025,China;Center for Microbiology and Biochemical Pharmaceutical Engineering,Guiyang 550025,China;Experimental Animal Center,Guizhou Medical University,Guiyang 550025,China;School of Pharmacy,Kunming Medical University,Kunming 650500,China)

机构地区：[1]省部共建药用植物功效与利用国家重点实验室/贵州医科大学药学院,贵阳550025 [2]贵州医科大学微生物与生化药学工程中心,贵阳550025 [3]贵州医科大学实验动物中心,贵阳550025 [4]昆明医科大学药学院,昆明650500

出　　处：《中国药房》2022年第17期2082-2086,2092,共6页China Pharmacy

基　　金：国家自然科学基金资助项目(No.81160413);抗宫炎软胶囊技术合作开发项目(No.2020702)。

摘　　要：目的 分析抗宫炎软胶囊(KSC)的质量标志物(Q-marker)。方法 采用超高效液相色谱(UPLC)法,建立20批KSC的指纹图谱,采用《中药色谱指纹图谱相似度评价系统(2012版)》进行相似度评价,确定共有峰;采用同一UPLC法测定去甲异波尔定、盐酸益母草碱、连翘酯苷B、毛蕊花糖苷、金石蚕苷、异毛蕊花糖苷的含量;通过网络药理学、分子对接方法筛选和分析KSC治疗宫颈炎的相关靶点和通路,构建“成分-靶点-通路”网络,分析其潜在Q-marker。结果 20批KSC共有12个共有峰,相似度均大于0.99;共指认出去甲异波尔定、盐酸益母草碱、连翘酯苷B、毛蕊花糖苷、金石蚕苷、异毛蕊花糖苷6个共有峰。上述6个成分的含量分别为1.336~1.774、0.093~0.143、4.970~5.888、0.505~0.623、5.206~6.226、0.785~0.895 mg/g。网络药理学筛选得到6个成分的14个关键靶点和94条通路,其与核心靶点(蛋白激酶B1、肿瘤坏死因子)的结合能均小于-6.4 kJ/mol。结论 去甲异波尔定、盐酸益母草碱等6个成分可能是KSC的潜在Q-marker。OBJECTIVE To analyze quality maker(Q-marker) of Kanggongyan soft capsule(KSC). METHODS The fingerprints of 20 batches of KSC were established by ultra high performance liquid chromatography(UPLC)method. Similarity Evaluation System of TCM Chromatographic Fingerprint(2012 edition)were used to evaluate the similarity and confirm common peaks. The contents of norisoboldine,leonurine hydrochloride,forsythoside B,acteoside,poliumoside and isoacteoside were determined by the same UPLC method. Targets and pathways related to KSC in the treatment of cervicitis were screened and analyzed by network pharmacology and molecular docking method to construct a “component-target-pathway” network, and analyze its potential Q-marker. RESULTS Twelve common peaks were identified in the fingerprints of 20 batches of KSC,and the similarity was greater than 0.99. Six common peaks were identified,including norisoboldine,leonurine hydrochloride,forsythoside B,acteoside,poliumoside and isoacteoside. The contents of the above 6 components were 1.336-1.774,0.093-0.143,4.970-5.888,0.505-0.623,5.206-6.226 and 0.785-0.895 mg/g,respectively. By network pharmacology analysis,14 key targets and 94 pathways were obtained,and their binding energies to the core targets(protein kinase B1,tumor necrosis factor)were all less than-6.4 k J/cal.CONCLUSIONS Six components such as norisoboldine and leonurine hydrochloride are potential Q-marker of KSC.

关 键 词：抗宫炎软胶囊 网络药理学 指纹图谱 含量测定 质量标志物 分子对接 

分 类 号：R917[医药卫生—药物分析学] R284[医药卫生—药学]