8种中药水提液对尖孢镰刀菌的抑制效果  

Antifungal effects of water extracts from 8 kinds of Chinese medicines on Fusarium oxysporum

作　　者：贾正燕 王昌梅[1,2] 张啸 赵兴玲 吴凯[1,2] 尹芳 杨斌 梁承月[1] 张无敌 JIA Zhengyan;WANG Changmei;ZHANG Xiao;ZHAO Xingling;WU Kai;YIN Fang;YANG Bin;LIANG Chengyue;ZHANG Wudi(School of Energy and Environmental Science,Yunnan Normal University,Kunming,Yunnan 650500,China;Jilin Dongsheng Biomass Energy Engineering Research Institute,Tonghua,Jilin 134118,China;Yunnan Kangyi Sanqi Planting Co.Ltd,Kunming,Yunnan 650032,China)

机构地区：[1]云南师范大学能源与环境科学学院,云南昆明650500 [2]吉林东晟生物质能工程研究院,吉林通化134118 [3]云南康艺三七种植有限公司,云南昆明650032

出　　处：《湖南农业大学学报（自然科学版）》2022年第4期454-459,共6页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：云南省发展改革委员会万人计划产业技术领军人才项目(20191096);吉林省科学技术厅项目(20200402099NC、20200403010SF);云南省科学技术厅国际科技合作专项(202003AF140001)。

摘　　要：为减少三七根腐病的发生,尝试采用8种中药(姜黄、茵陈、金银花、马齿苋、黄芩、紫花地丁、黄连、黄柏)水提液对三七根腐病原菌中尖孢镰刀菌进行抑制,采用二倍稀释法测定8种中药对尖孢镰刀菌的最小抑菌浓度,采用生长速率法测定中药水提液对尖孢镰刀菌的抑菌活性。结果表明:8种中药水提液对尖孢镰刀菌均有抑制效果,其中,金银花水提液对尖孢镰刀菌的抑菌效果最佳,最大抑菌率可达75%,EC50最小,为5.55 mg/mL;黄连、金银花、黄芩对尖孢镰刀菌的MIC≤1 mg/mL,说明其对尖孢镰刀菌的有效起始抑菌浓度较低。In order to reduce the occurrence of root rot of Panax notoginseng(P.notoginseng),the water extracts of 8 traditional Chinese medicines(Curcuma longa,Artemisia capillaris,Lonicerae japonicae,Portulaca oleracea,Scutellaria baicalensis,Viola yedoensis,Coptis chinensis,Phellodendron chinense)were used to inhibit Fusarium oxysporum(F.oxysporum),one of the pathogens of root rot of P.notoginseng.The minimum inhibitory concentration(MIC)of 8 traditional Chinese medicines against F.oxysporum was determined by double dilution method,and the antifungal activity of water extracts of Chinese medicines against F.oxysporum was determined by growth rate method.The results showed that the water extracts of 8 kinds of traditional Chinese medicines had inhibitory effects on F.oxysporum.Among them,the water extract of L.japonicae has the best fungicidal effect on F.oxysporum,the maximum fungicidal rate was 75%and the EC50 was the lowest being 5.55 mg/mL.Coptis chinensis,Lonicerae japonicae and Scutellaria baicalensis all showed MIC≤1 mg/mL against F.oxysporum,indicating their effective initial antifungal concentration against F.oxysporum was low.

关 键 词：三七根腐病 中药水提液 尖孢镰刀菌 毒力 抑菌活性 

分 类 号：S435.67[农业科学—农业昆虫与害虫防治]