砷胁迫对青萍PSⅡ荧光参数和光响应的影响  

Effects of arsenic stress on PSⅡfluorescence parameters and light response of Lemna minor L

作　　者：钟智遥 胡良 王香莲[3] 高桂青[3] 樊后保[1,2] 孙珑[1,2] 胡晨阳 胡盛明 ZHONG Zhiyao;HU Liang;WANG Xianglian;GAO Guiqing;FAN Houbao;SUN Long;HU Chenyang;HU Shengming(School of Hydraulic and Ecological Engineering,Nanchang 330099,China;Jiangxi Key Laboratory for Restoration of Degraded Ecosystems&Watershed Ecohydrology,Nanchang 330099,China;School of Civil and Architectural Engineering,Nanchang Institute of Technology,Nanchang 330099,China)

机构地区：[1]南昌工程学院水利与生态工程学院,江西南昌330099 [2]南昌工程学院江西省退化生态系统修复与流域生态水文重点实验室,江西南昌330099 [3]南昌工程学院土木与建筑工程学院,江西南昌330099

出　　处：《南昌工程学院学报》2022年第4期23-28,36,共7页Journal of Nanchang Institute of Technology

基　　金：江西省自然科学基金资助项目(20212BAB204042);国家自然科学基金资助项目(42162023);江西省教育厅科学技术研究项目(GJJ201904);江西省科技重点研发计划项目(20203BBGL73220)。

摘　　要：通过水培试验,以青萍(Lemna minor L)为研究材料,研究不同浓度砷胁迫对青萍生物量、荧光参数、快速光响应和砷富集量的影响。结果显示,随着砷处理浓度增加,青萍生物量、有效量子产量Y(Ⅱ)、潜在光化学效率(F_(v)/F_(o))、最大光化学效率(F_(v)/F_(m))和光化学荧光淬灭系数(q_(P))显著下降(P<0.05),非调节性能量耗散量子产量Y(NO)、调节性能量耗散量子产量Y(NPQ)和砷单位富集量显著上升(P<0.05),非光化学淬灭系数(q_(N))先升后降。电子传递效率(ETR)随着砷处理浓度增大,呈显著下降的趋势;然而当有效辐射强度(PAR)增大时,ETR呈先升后逐渐趋于平稳的趋势。本研究表明,青萍在受到砷胁迫时,可通过调节PSⅡ反应中心进行自我保护,缓解砷毒害;对于低浓度(As^(3+)≤3mg·L^(-1))时,则表现出一定耐受性,且砷浓度越高,青萍对砷单位富集量越大。然而,当为高砷处理浓度时,则产生严重抑制作用。本研究可为青萍修复As^(3+)污染水体提供参考意义。Through hydroponic experiments,using Lemna minor L as the research material,the effects of arsenic stress on biomass,fluorescence parameters,rapid light response and arsenic content of Lemna minor were studied.The results show that with the increase of the concentration of arsenic treatment,the biomass,effective quantum yield(YⅡ),potential photochemical efficiency(F_(v)/F_(o)),maximum photochemical efficiency(F_(v)/F_(m))and photochemical fluorescence quenching coefficient(q_(P))decreased significantly as the concentration of arsenic treatment increased.(P<0.05);that non-regulated energy dissipation quantum yield(YNO),regulated energy dissipation quantum yield(YNPQ)and arsenic content increased significantly(P<0.05),and non-photochemical quenching coefficient(q_(N))first rise and then fall.The electron transfer efficiency(ETR)showed a significant downward trend as the concentration of arsenic treatment increased,and as the effective radiation intensity(PAR)increased,the ETR increased first and then gradually stabilized.The above results show that when arsenic is stressed,Lemna minor can protect itself by adjusting the PSⅡreaction center to alleviate arsenic toxicity;for low concentrations(As^(3+)≤3mg·L^(-1)),it shows certain tolerance,high concentration of arsenic has a serious inhibitory effect at the concentration.And the higher the concentration of arsenic,the higher the concentration of arsenic per unit by Lemna minor.This study can provide reference for Lemna minor to repair As^(3+)polluted water bodies.

关 键 词：青萍 荧光参数 PSⅡ反应中心 砷 

分 类 号：X53[环境科学与工程—环境工程]