细胞膜力学性能对电穿孔影响的仿真研究  

Simulation Study on the Effect of Cell Membrane Mechanical Properties on Electroporation

作　　者：米彦[1] 吴晓 徐进[1] 郑伟[1] 马驰 Mi Yan;Wu Xiao;Xu Jin;Zheng Wei;Ma Chi(State Key Laboratory of Power Transmission Equipment&System Security and New Technology Chongqing University,Chongqing 400030,China)

机构地区：[1]输配电装备及系统安全与新技术国家重点实验室(重庆大学),重庆400030

出　　处：《电工技术学报》2022年第17期4326-4334,4400,共10页Transactions of China Electrotechnical Society

基　　金：国家自然科学基金(52077022,51477022);“111”引智项目(BP0820005)资助。

摘　　要：为探究细胞膜的力学性能对电穿孔的影响,该文将细胞膜视为一种超弹性材料,在COMSOL中建立细胞膜上局部单个微孔的机电耦合模型,分析细胞膜弹性应变能对微孔形成的影响,并比较了细胞膜弹性模量不同时微孔形成过程的差异。结果表明,细胞膜的应变能一方面增大了从疏水孔向亲水孔转变时的越过能垒,并且膜弹性模量越大,越过能垒越大,且二者线性相关;另一方面,应变能的增加使微孔稳定,阻止微孔无限扩大,稳定尺寸与细胞膜力学性能和跨膜电位有关。该文通过仿真揭示了细胞膜的力学性能在电穿孔中的重要作用,并为研究细胞结构对电穿孔的影响机制提供了理论参考。To explore the influence of the mechanical properties of cell membrane on electroporation,cell membrane was regarded as a hyperelastic material,and an electromechanical coupling model of local single pore on the cell membrane was established in COMSOL.The influence of the elastic strain energy of the cell membrane on the pore formation was analyzed and the difference of pore formation with different elastic modulus of the cell membrane was compared.The results show that the strain energy of the cell membrane increases the energy barrier for transition from hydrophobic pore to hydrophilic pore.The greater the elastic modulus of the cell membrane,the greater the energy barrier is,and the two are linearly related.On the other hand,the increased strain energy stabilizes the pores and prevents them from expanding indefinitely,and the stable size is related to the mechanical properties of the cell membrane and the transmembrane potential.This paper reveals the importance role of the mechanical properties of the cell membrane in electroporation through simulation,which provides a theoretical reference for studying the influence mechanism of cell structure on electroporation.

关 键 词：力学性能 电穿孔 机电耦合 应变能 微孔能量 

分 类 号：TM836[电气工程—高电压与绝缘技术] Q64[生物学—生物物理学]