马铃薯锌转运体基因StZnT2生物信息学及表达特性分析  

Bioinformatics and Expression Characteristic Analysis of Zinc Transporter StZnT2 Gene of Potato(Solanum tuberosum)

作　　者：尹科 赵婧[3] 唐勋 赵贵宾 朱永永 党伟英 张宁[1,2] 司怀军 YIN Ke;ZHAO Jing;TANG Xun;ZHAO Gui-Bin;ZHU Yong-Yong;DANG Wei-Ying;ZHANG Ning;SI Huai-Jun(College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070,China;Gansu Provincial Key Laboratory of Arid-land Crop Science,Lanzhou 730070,China;Gansu Agricultural Information Center,Lanzhou 730030,China;Gansu General Station of Agrotechnology Extension,Lanzhou 730020,China)

机构地区：[1]甘肃农业大学生命科学技术学院,兰州730070 [2]甘肃省干旱生境作物学省部共建国家重点实验室,兰州730070 [3]甘肃省农业信息中心,兰州730030 [4]甘肃省农业技术推广总站,兰州730020

出　　处：《农业生物技术学报》2022年第9期1676-1686,共11页Journal of Agricultural Biotechnology

基　　金：甘肃富锌马铃薯生产技术集成与应用项目(GNKJ-2021-25);甘肃省马铃薯绿色高质高效创建综合配套技术集成与研究应用项目(GNKJ-2021-26);联合国世界粮食计划署项目(WFPGSPP-1)。

摘　　要：锌是植物生长发育所必需的微量元素,缺锌或过量锌都会严重影响植物生长,锌转运蛋白对调节植物体内锌稳态具有重要作用。本研究以马铃薯(Solanum tuberosum)栽培品种’大西洋’试管苗为实验材料,克隆得到马铃薯锌转运体2(Solanum tuberosum zinc transporter 2,StZnT2)(GenBank No.XM_006358632.2)基因。结果表明,该基因全长1294 bp,CDS长1011 bp,编码336个氨基酸,蛋白相对分子量为36682.78 kD,理论等电点为5.63,属于疏水性跨膜蛋白,具有典型的ZIP保守结构域;亚细胞定位结果显示该蛋白定位于细胞膜上。同源蛋白比对分析表明,StZnT2基因编码的蛋白质与潘那利番茄(S.pennellii)、番茄(S.lycopersicum)和科民茄(S.commersonii)相似度最高。马铃薯在缺乏锌和过量锌培养条件下均表现出植株矮小、根系伸长受到抑制。实时荧光定量PCR分析不同锌浓度胁迫下基因的表达模式,结果表明,StZnT2在缺锌和过量锌胁迫下表达量均上调,且在根中表达水平最高。本研究结果为进一步阐明马铃薯StZnT2基因功能提供理论依据。Zinc is a trace element necessary for plant growth and development.The lacking or presence of excessive zinc have severe impacts on growth of plants.Zinc transporter plays an important role in regulating zinc homeostasis in plants.In this study,the potato(Solanum tuberosum)zinc transporter gene StZnT2(GenBank No.Xm_006358632.2)was cloned from the in vitro seedlings of potato cultivar’Atlantic’.The results showed that the gene was 1294 bp in length and 1011 bp in CDS region,encoding 336 amino acids.Protein molecular weight was 36682.78 kD.Theoretical isoelectric point was 5.63.StZnT2 was a hydrophobic transmembrane protein with a typical ZIP conserved domain.StZnT2 was located on the cell membrane.Comparative analysis of homologous proteins showed that the proteins encoded by StZnT2 gene were most similar to S.pennellii,S.lycopersicum and S.commersonii.Potato plants were stunted and root elongation was inhibited under zinc deficiency and zinc toxicity.The expression of genes under different zinc concentration gradient stress were analyzed by qPCR.The results showed that the expression level of StZnT2 was up-regulated under zinc deficiency and excessive zinc stress,and the expression level was the highest in roots.These results provide theoretical basis for further elucidating the function of StZnT2 gene in potato.

关 键 词：锌 马铃薯 马铃薯锌转运体2(StZnT2) 生物信息学 表达模式 

分 类 号：S532[农业科学—作物学]