NLRC5基因多态性与胃癌易感性的关系  

Relationship between NLRC5 gene polymorphisms and gastric cancer susceptibility

作　　者：倪栋琼 谭惠城 张欣怡 邵欢 黄宣[2] Dong-Qiong Ni;Hui-Cheng Tan;Xin-Yi Zhang;Huan Shao;Xuan Huang(Department of Gastroenterology,Affiliated Hospital of Shaoxing University,Shaoxing 312000,Zhejiang Province,China;Department of Gastroenterology,The First Affiliated Hospital,Zhejiang Chinese Medical University,Hangzhou 310006,Zhejiang Province,China)

机构地区：[1]绍兴文理学院附属医院浙江省绍兴市,312000 [2]浙江中医药大学附属第一医院,浙江省杭州市310006

出　　处：《世界华人消化杂志》2022年第16期701-709,共9页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目,No.81673745;浙江省医药卫生科技计划,No.2020RC025。

摘　　要：背景 NF-κB信号通路对胃癌的发生起协同作用,而NLRC5作为其上游因子,与胃癌的发生密切相关.但是NLRC5在不同胃癌患者中的表达不同,可能与NLRC5基因多态性有关,我们通过基因测序来研究NLRC5基因多态性与胃癌的关系.目的 探讨NLRC5基因多态性与胃癌易感性及胃癌患者预后的关系.方法 选用2014-09/2016-10浙江中医药大学附属第一医院胃癌患者75例和同期健康人群59例(年龄和性别匹配)作为研究对象,采用PCR产物一代测序法检测NLRC5rs56315364和rs289726基因型,测序产物采用MegA lign和Chromas2.4.3软件进行分析,比较胃癌患者和健康人群NLRC5基因多态性与胃癌患者预后的关系.结果 NLRC5rs56315364CC基因型增加胃癌发病风险(OR=7.06,95%CI:2.81-17.72),rs289726TC、CC基因型增加胃癌发病风险(OR=11.04,95%CI:4.29-28.43;OR=4.77,95%CI=1.57-14.48),且幽门螺杆菌(Helicobacter pylori,H.pylori)阴性和阳性组间无显著差异(P>0.05).生存分析结果 显示NLRC5rs289726基因型与胃癌预后有关(P<0.05),且CC基因型的胃癌患者预后最差.COX多因素回归分析发现年龄和TNM分期与胃癌患者预后显著相关(P<0.05).结论 NLRC5rs56315364CC基因型和NLRC5rs289726TC基因型增加胃癌的发病风险.年龄越大、TNM分期越高,胃癌患者预后越差,NLRC5 rs289726携带CC基因型的胃癌患者预后最差.BACKGROUND The NF-κB signaling pathway exerts a synergistic effect on gastric carcinogenesis.NLRC5 is an upstream regulator of the NF-κB signaling pathway,which is closely related to gastric carcinogenesis.Since NLRC5 expression is different among gastric cancer patients,we speculated that it might be related to NLRC5 gene polymorphisms.We explored the associations between NLRC5 gene polymorphism and gastric carcinogenesis through gene sequencing.AIM To investigate the associations of the NLRC5 rs56315364 and rs289726 gene polymorphisms with gastric cancer susceptibility and prognosis.METHODS A total of 75 gastric cancer patients and 59 healthy volunteers(age-and sex-matched) were enrolled in the study from September 2014 to October 2016.The NLRC5 rs56315364 and rs289726 genotypes were determined by first-generation sequencing of PCR products.Sequencing products were analyzed using MegAlign and Chromas 2.4.3 software.Differences in NLRC5 gene polymorphisms between patients with gastric cancer and healthy volunteers were identified to investigate the relationship between NLRC5 gene polymorphisms and the prognosis of gastric cancer.RESULTS The NLRC5 rs56315364 CC genotype increased the risk of gastric cancer [odds ratio(OR) = 7.06,95% confidence interval(CI):2.81-17.72],as did the rs289726 TC and CC genotypes(OR = 11.04,95%CI:4.29-28.43;OR = 4.77,95%CI:1.57-14.48,respectively).There was no significant difference in the genotype frequency of NLRC5 rs56315364 and rs289726 between the Helicobacter pylori(H.pylori)-negative group and the H.pylori-positive group(P > 0.05).Survival analysis showed that the NLRC5 rs289726 genotype was correlated with the prognosis of gastric cancer(P < 0.05),and the NLRC5 rs289726 CC genotype was associated with the worst prognosis.Multivariate Cox regression analysis showed that age and tumor-nodemetastasis(TNM) stage were correlated with the prognosis of gastric cancer patients(P < 0.05) CONCLUSION The NLRC5 rs56315364 CC and rs289726 TC genotypes significantly increase the risk of ga

关 键 词：NLRC5 基因多态性 胃癌 幽门螺杆菌 预后 

分 类 号：R735.2[医药卫生—肿瘤]