高效液相色谱-串联质谱法测定食用动物组织中那西肽残留  

Determination of nosiheptide residue in food animal tissues by high performance liquid chromatography-tandem mass spectrometry

作　　者：聂晨 韩静静 赵飒 杨帆 何京澄 王培锋 李明哲 林超 张鸿伟 NIE Chen;HAN Jingjing;ZHAO Sa;YANG Fan;HE Jingcheng;WANG Peifeng;LI Mingzhe;LIN Chao;ZHANG Hongwei(Qingdao Customs Technology Center,Shandong Qingdao 266002,China;School of Food Science and Engineering,Ocean University of China,Shandong Qingdao 266002,China)

机构地区：[1]青岛海关技术中心,山东青岛266002 [2]中国海洋大学食品科学与工程学院,山东青岛266002

出　　处：《中国食品卫生杂志》2022年第4期755-760,共6页Chinese Journal of Food Hygiene

基　　金：青岛海关科技项目(QK202017)。

摘　　要：目的 建立高效液相色谱-串联质谱法测定食用动物组织(猪肉、猪肝脏、猪肾脏、鸡肉、鸡肝脏及鸡肾脏)中那西肽残留含量。方法 动物组织中那西肽残留经0.1%甲酸乙腈提取,Captiva ND固相萃取柱净化,液相色谱-串联质谱多反应监测负离子模式扫描测定,基质匹配标准曲线外标法定量。结果 各相关基质中那西肽在2~200μg/L浓度范围内线性关系良好,相关系数r>0.99,各动物组织中的检出限为2.0μg/kg,定量限为7.0μg/kg。在3.5、7.0和70μg/kg添加浓度下,那西肽的回收率范围为76.27%~92.31%,相对标准偏差为2.15%~8.03%。结论 该方法简便快速、准确度高和重复性良好,适用于食用动物组织中那西肽残留的监测与定量测定。Objective To develop a method for the determination of nosiheptide residue in food animal tissues(porcine muscle,porcine liver,porcine kidney,chicken muscle,chicken liver and chicken kidney)with high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).Methods After extracted by 0. 1% acidic acetonitrile,the nosiheptide extract was cleaned up by Captiva ND cartridge and determined by HPLC-MS/MS in negative electrospray ionization mode. Quantification was performed using corresponding matrix-matched calibration curves.Results Nosiheptide in corresponding matrix showed good linear relationships(r>0. 99)in the calibration range of 2~200 μg/L,and the limit of detection(LOD)and limit of quantification(LOQ)of the method were 2. 0 and 7. 0 μg/kg,respectively. The recoveries of the method ranged from 76. 27% to 92. 31% at the spiking level of 3. 5,7. 0 and 70 μg/kg with relative standard deviations of 2. 15%~8. 03%.Conclusion The proposed method is simple,rapid,accurate as well as reproducible and can be applied to monitor and determine nosiheptide residue in food animal tissues.

关 键 词：高效液相色谱-串联质谱法 那西肽 食用动物组织 

分 类 号：R155[医药卫生—营养与食品卫生学]