2018—2020年北京市海淀区腹泻患者弯曲菌流行特征及PFGE分型研究  

Analysis on epidemiological characteristics and PFGE typing of Campylobacter in diarrhea patients in Haidian District of Beijing from 2018 to 2020

作　　者：白婧[1] 谢利军[1] 郝春芬 江泽芬[1] BAI Jing;XIE Lijun;HAO Chunfen;JIANG Zefen(Microbiological Laboratory,Beijing Haidian District Center for Disease Control and prevention,Beijing 100094,China)

机构地区：[1]北京市海淀区疾病预防控制中心微生物检验科,北京100094

出　　处：《中国食品卫生杂志》2022年第4期830-835,共6页Chinese Journal of Food Hygiene

摘　　要：目的 分析和掌握2018-2020年北京市海淀区腹泻患者中弯曲菌流行特征和脉冲场凝胶电泳(PFGE)分型情况。方法 对2018-2020年在3家哨点医院就诊的756例腹泻患者便标本进行弯曲菌的分离和鉴定,对其中25株弯曲菌菌株进行PFGE分型试验。结果 弯曲菌总体检出率为4.37%(33/756),空肠弯曲菌和结肠弯曲菌检出率分别为3.04%(23/756)和1.32%(10/756);不同性别、年龄和职业之间的弯曲菌检出率比较,差异均无统计学意义(P>0.05);48.48%(16/33)的弯曲菌感染患者出现了发热,弯曲菌患者大便性状以水样便为主。弯曲菌检出主要集中在5~7月,除不明食品外,可疑暴露食品占比最高的为肉与肉制品。17株空肠弯曲菌聚类分析得到16种带型,8株结肠弯曲菌聚类分析得到7种带型。结论 海淀区弯曲菌污染状况不容乐观,本研究填补了北京市海淀区腹泻患者弯曲菌感染状况数据的空白,为海淀区食源性疾病的预防和控制提供了参考依据。Objective To analyze and master the epidemiological characteristics and pulsed-field gel electrophoresis(PFGE)typing of Campylobacter in diarrhea patients in Haidian District of Beijing from 2018 to 2020.Methods Stool samples of 756 diarrhea patients were collected in 3 hospitals from 2018 to 2020. Campylobacter was isolated and identified,the isolated 25 Campylobacter strains were analyzed by PFGE for genetic typing.Results The total detection rate of Campylobacter was 4. 37%(33/756),of which the detection rates of Campylobacter jejuni(C. jejuni)and Campylobacter coli(C. coli)were 3. 04%(23/756)and 1. 32%(10/756),respectively. Epidemiological characteristics regarding the heterogeneities of Campylobacter showed no significant differences among different gender,age,and occupation. 48. 48%(16/33)diarrhea patients experienced acute watery diarrhea,and they usually have a fever. The peak of detection rate sustained from May to July. Meat and meat products were recognized as a primary source of food-related transmission of Campylobacter to human. 16 and 7 patterns were detected through PFGE in C. jejuni strains and C. coli strains,respectively.Conclution Campylobactercontamination deserved more attention. This study fills the data gap of Campylobacter infection in diarrhea patients in Haidian District of Beijing,and provides references for the prevention and control of foodborne diseases in Haidian District.

关 键 词：腹泻患者 弯曲菌 检出率 流行特征 分子分型 

分 类 号：R155[医药卫生—营养与食品卫生学]