骨碎补总黄酮对亚硝酸钠和东莨菪碱所致记忆障碍小鼠下丘脑区形态和雌激素受体表达的影响  

Effects of total flavonoids of Drynariae Rhizoma on morphology and estrogen receptor expression in hypothalamus of memory impairment mice induced by sodium nitrite and scopolamine

作　　者：孙婷 崔悦 赵晨宇 刘彦宏 徐红丹[1,4] 孙慧峰[1] 杨波[1] 张宁[1,4] SUN Ting;CUI Yue;ZHAO Chen-yu;LIU Yan-hong;XU Hong-dan;SUN Hui-feng;YANG Bo;ZHANG Ning(School of Pharmacy,Heilongjiang University of Chinese Medicine,Harbin 150040,China;School of Pharmacy,Jiamusi University,Jiamusi 154007,China;School of Basic Medicine,Heilongjiang University of Chinese Medicine,Harbin 150040,China;Jiamusi College,Heilongjiang University of Chinese Medicine,Jiamusi 154007,China)

机构地区：[1]黑龙江中医药大学药学院,哈尔滨150040 [2]佳木斯大学药学院,佳木斯154007 [3]黑龙江中医药大学基础医学院,哈尔滨150040 [4]黑龙江中医药大学佳木斯学院,佳木斯154007

出　　处：《中华中医药杂志》2022年第8期4418-4422,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金面上项目(No.81673581);国家科技重大专项(No.2018ZX09201009);黑龙江省属高校科技成果研发、培育项目(No.TSTAU-C2018020);黑龙江中医药大学科研基金项目(No.2018pt04,No.2018RCD19,No.2019BJP01)。

摘　　要：目的:研究骨碎补总黄酮对记忆障碍模型小鼠学习记忆能力及雌激素受体(ER)表达的影响。方法:对亚硝酸钠和东莨菪碱两种记忆障碍模型小鼠给予骨碎补总黄酮进行干预,采用跳台实验观察小鼠的学习及记忆能力;采用HE染色法观察小鼠下丘脑的病理变化,免疫组化法观察小鼠下丘脑中ERα表达。结果:与模型组比较,各给药组小鼠潜伏期显著延长(P<0.01),错误次数显著减少(P<0.01),下丘脑ERα的表达显著增加(P<0.01);HE染色显示,模型组小鼠神经细胞排列得比较紧密,细胞间距变大,细胞核染色较深,核仁较为清晰。结论:骨碎补总黄酮使两种记忆障碍模型小鼠的学习记忆能力得到改善,其作用机制可能通过调节下丘脑区ER表达发挥神经保护作用。Objective:To study the effects of total flavonoids of Drynariae Rhizoma on learning,memory ability and estrogen receptor(ER)expression in mice with memory impairment.Methods:Sodium nitrite and scopolamine were given to mice with two kinds of memory impairment models to intervene with total flavonoids of Drynariae Rhizoma,and the learning and memory abilities of mice were observed by step-down test.HE staining was used to observe the pathological changes in the hypothalamus of mice,and the expression of ERαin the hypothalamus of mice was observed by immunohistochemistry.Results:Compared with the model group,the incubation period of mice in each administration group was significantly prolonged(P<0.01),the number of errors was significantly decreased(P<0.01),and the expression of hypothalamic ERαwas significantly increased(P<0.01).HE staining showed that the nerve cells in the model group were arranged closely,the cell spacing became larger,the nucleus staining was deeper and the nucleolus was clearer.Conclusion:The total flavonoids of Drynariae Rhizoma improved the learning and memory abilities of mice with two kinds of memory impairment models,and its mechanism may play a neuroprotective role by regulating the expression of ER in hypothalamus.

关 键 词：阿尔茨海默病 骨碎补总黄酮 植物雌激素 亚硝酸钠 东莨菪碱 记忆障碍 

分 类 号：R285.5[医药卫生—中药学]