游离氨对生物脱氮硝化过程细菌种群结构的影响  

Effect of free ammonia on microbial community structure in biological nitrification process

作　　者：董昆 李奕燃 李晓强[2] 王光杰 孙洪伟 DONG Kun;LI Yiran;LI Xiaoqiang;WANG Guangjie;SUN Hongwei(School of Environmental and Municipal Engineering,Lanzhou Iiaotong University,Lanzhou,730070,China;School of Environmental and Material Engineering,Yantai University,Yantai,264000,China;Shandong Tongji Testing Technology Co.Ltd.,Yantai,264000,China)

机构地区：[1]兰州交通大学环境与市政工程学院,兰州730070 [2]烟台大学环境与材料工程学院,烟台264000 [3]山东同济测试科技股份有限公司,烟台264000

出　　处：《环境化学》2022年第8期2742-2751,共10页Environmental Chemistry

基　　金：国家自然科学基金(51668031)资助。

摘　　要：为揭示游离氨(FA)对硝化过程影响的生物学机制,本试验以人工模拟废水为研究对象,采用4组平行的SBR反应器(R_(0.5)、R_(5)、R_(10)和R_(15)),基于16S r RNA基因-Illumina Mi Seq高通量测序技术,考察了4种FA浓度(0.5、5、10、15 mg·L^(-1))对SBR反应器中的细菌种群结构的影响.结果表明,FA会显著影响系统内的微生物多样性和菌群结构.R_(0.5)的Chao1、ACE、Shannon和Simpson指数均为最大,其具有最高的微生物多样性,而R15的微生物多样性最低.在微生物门水平上,最优势菌门变形菌门(Proteobacteria)的相对丰度与FA浓度呈正相关,硝化螺旋菌门(Nitrospirae)的相对丰度在R15中最低.在微生物属水平上,亚硝化单胞菌属(Nitrosomonas)和硝化螺旋菌属(Nitrospira)的相对丰度在R10中显著较高,动胶菌属(Zoogloea)和陶厄氏菌属(Thauera)的相对丰度与FA浓度呈显著的线性相关.基于LEf Se分析共获得了24种具有显著差异的微生物,从而得到了4种FA浓度条件下的关键微生物标记物.本研究加深了对生物脱氮硝化过程菌群结构的认识,为深入研究生物脱氮硝化的抑制机理提供了借鉴.Based on 16 S rRNA genes-Illumina Mi Seq high-throughput sequencing,this study is to investigate the community and structure of characteristic microbial communities related to nitrification under four FA concentrations(0.5,5,10,15 mg·L^(-1)) settled in four parallel laboratoryscale sequencing batch reactors(SBRs,denoted as R_(0.5)、R_(5)、R_(10)、R_(15)) to reveal the biological mechanism of how does FA influence the nitrification.Results indicated that FA concentration significantly affects the microbial diversity and community structure in the system.The largest α-diversity values of Chao1,ACE,Shannon and Simpson were achieved in R_(0.5),which represents with highest diversity of bacterial community,while the lowest was achieved in R_(15).At the the phylum level,the relative abundance of Proteobacteria which is the predominant phylum,increased with the increasing of FA concentration.The relative abundance of Nitrospira in the nitrification process was the lowest in R_(15).At the genus level,the relative abundance of Nitrosomonas and Nitrospira was significantly higher in R_(10).The relative abundance of Zoogloea and Thauera showed a significant linear correlation with FA concentration.A total of 25 groups of microorganisms with significant differences were obtained based on LEf Se analysis,thereby key biomarkers of the microflora were obtained at the microbiological classification level under four FA concentrations.This study has deepened the understanding of the microbial community structure in the biological nitrification process,and provided a reference for in-depth study of the inhibition mechanism of biological nitrification process.

关 键 词：游离氨 Illumina MiSeq高通量测序 硝化过程 微生物多样性 LEfSe组间差异 

分 类 号：X703[环境科学与工程—环境工程] X172