溶质载体家族39A14对弥漫大B细胞淋巴瘤OCI-LY3细胞增殖、迁移和侵袭的影响  

Effects of solute carrier family 39A14 on proliferation,migration and invasion of diffuse large B-cell lymphoma OCI-LY3 cells

作　　者：白敏[1] 黄云鹏[1] 关涛[1] 王列样[1] 苏丽萍[1] Bai Min;Huang Yunpeng;Guan Tao;Wang Lieyang;Su Liping(Department of Hematology,Shanxi Province Cancer Hospital,Shanxi Hospital Affiliated to Cancer Hospital,Chinese Academy of Medical Sciences,Cancer Hospital Affiliated to Shanxi Medical University,Taiyuan 030013,China)

机构地区：[1]山西省肿瘤医院,中国医学科学院肿瘤医院山西医院,山西医科大学附属肿瘤医院血液科,太原030013

出　　处：《肿瘤研究与临床》2022年第7期521-524,共4页Cancer Research and Clinic

摘　　要：目的探讨溶质载体家族39(SLC39)A14对弥漫大B细胞淋巴瘤(DLBCL)OCI-LY3细胞增殖、迁移和侵袭的影响。方法将人DLBCL细胞株OCI-LY3分为Vector组(瞬时转染空载对照质粒)和SLC39A14组(转染SLC39A14质粒)。采用CCK-8法检测两组OCI-LY3细胞增殖情况,Transwell法检测细胞迁移和侵袭情况,蛋白质印迹法检测OCI-LY3细胞SLC39A14蛋白表达水平以及PI3K-AKT-mTOR信号通路相关蛋白表达情况。结果与Vector组相比,第3天至第5天SLC39A14组细胞增殖能力均增加(均P<0.05)。Transwell细胞迁移实验结果显示,36 h后Vector组迁移细胞数为(64±4)个,SLC39A14组为(236±25)个,SLC39A14组细胞迁移能力增强,差异有统计学意义(t=15.02,P<0.05);侵袭实验结果显示,Vector组侵袭细胞数为(32±2)个,SLC39A14组为(127±17)个,SLC39A14组细胞侵袭能力增强,差异有统计学意义(t=8.33,P<0.05)。蛋白质印迹法检测结果显示,SLC39A14组pmTOR、pAKT、pPI3K蛋白表达水平均升高。结论 SLC39A14可能通过PI3K-AKT-mTOR信号通路参与DLBCL发生、发展。Objective To investigate the effects of solute carrier family 39(SLC39)A14 on proliferation,migration and invasion of diffuse large B-cell lymphoma(DLBCL)OCI-LY3 cells.Methods The human DLBCL cell line OCI-LY3 was divided into Vector group(transfected with empty control plasmid)and SLC39A14 group(transfected with SLC39A14 plasmid).The proliferation of OCI-LY3 cells in the two groups was detected by CCK-8 method,the migration and invasion of cells were detected by Transwell method,and the expression level of SLC39A14 protein and the expressions of PI3K-AKT-mTOR signaling pathway-related proteins in OCI-LY3 cells were detected by Western blotting.Results Compared with the Vector group,the cell proliferation ability in the SLC39A14 group was increased from day 3 to day 5(all P<0.05).The results of Transwell cell migration assay showed that the number of migrating cells after 36 h in the Vector group was(64±4)cells,and that in the SLC39A14 group was(236±25)cells.The cell migration ability in the SLC39A14 group was increased,and the difference was statistically significant(t=15.02,P<0.05).The results of Transwell cell invasion assay showed that the number of invasive cells in the Vector group was(32±2)cells,and that in the SLC39A14 group was(127±17)cells.The cell invasion ability in the SLC39A14 group was increased,and the difference was statistically significant(t=8.33,P<0.05).The results of Western blotting showed that the expression levels of pmTOR,pAKT and pPI3K proteins in the SLC39A14 group were all increased.Conclusions SLC39A14 may be involved in the occurrence and development of DLBCL through PI3K-AKT-mTOR signaling pathway.

关 键 词：淋巴瘤 大B-细胞 弥漫性 溶质载体家族39成员14 细胞增殖 细胞迁移 细胞侵袭 

分 类 号：R733.1[医药卫生—肿瘤]