基质金属蛋白酶-11对骨肉瘤细胞上皮-间质转化的调控作用  

Role of matrix metalloproteinase-11 in regulating epithelial-mesenchymal transition of osteosarcoma cells

作　　者：徐江波[1] 翁友林 蔡昱 郭彬 阿布都艾尼·热吾提 XU Jiang-bo;WENG You-lin;CAI Yu;GUO Bin;Abuduaini REWUTI(Department of Orthopedic Trauma,People's Hospital of Xinjiang Uygur Autonomous Region,Urumqi,Xinjiang Uygur Autonomous Region 830000,China;Graduate School,Xinjiang Medical University,Urumqi Xinjiang Uygur Autonomous Region 830000,China)

机构地区：[1]新疆维吾尔自治区人民医院骨科创伤病区,新疆维吾尔自治区乌鲁木齐83000 [2]新疆医科大学研究生学院,新疆维吾尔自治区乌鲁木齐830000

出　　处：《中华实用诊断与治疗杂志》2022年第8期765-768,共4页Journal of Chinese Practical Diagnosis and Therapy

基　　金：新疆维吾尔自治区自然科学基金面上项目(2019D01C127)。

摘　　要：目的观察基质金属蛋白酶-11(matrix metalloproteinase-11,MMP-11)对骨肉瘤细胞增殖、侵袭、转移的影响,探讨其对骨肉瘤细胞上皮-间质转化的调控作用。方法对数生长期Saos-2细胞随机分为空白对照组(正常培养)、siRNA-MMP-11组(转染siRNA-MMP-11)、ovRNA-MMP-11组(转染ovRNA-MMP-11)。转染48 h采用实时荧光定量PCR法检测3组细胞MMP-11 mRNA相对表达量,采用Western blot法检测细胞E-cadherin、N-cadherin、MMP-11蛋白相对表达量,采用Transwell小室实验检测侵袭细胞数,采用划痕实验检测迁移细胞数;采用MTT法检测转染后培养72 h时3组细胞增殖率。结果转染48 h,ovRNA-MMP-11组MMP-11 mRNA和蛋白相对表达量(1.511±0.082、1.688±0.115)均高于空白对照组(1.339±0.211、1.024±0.066)、siRNA-MMP-11组(1.147±0.382、0.382±0.068)(P<0.05),空白对照组均高于siRNA-MMP-11组(P<0.05);ovRNA-MMP-11组迁移细胞数、侵袭细胞数[(35.528±9.241)、(352.807±63.715)个]均多于siRNA-MMP-11组[(21.330±1.627)、(174.265±50.242)个]、空白对照组[(12.330±0.577)、(335.421±140.261)个](P<0.05),siRNA-MMP-11组迁移细胞数多于空白对照组(P<0.05),侵袭细胞数少于空白对照组(P<0.05);ovRNA-MMP-11组细胞E-cadherin蛋白相对表达量(0.557±0.236)低于空白对照组(0.653±0.202)、siRNA-MMP-11组(0.905±0.324)(P<0.05),空白对照组低于siRNA-MMP-11组(P<0.05);3组细胞N-cadherin蛋白相对表达量比较差异无统计学意义(P>0.05)。转染后培养72 h,ovRNA-MMP-11组细胞增殖率[(84.1±11.3)%]高于空白对照组[(76.3±7.3)%]、siRNA-MMP-11组[(57.0±7.1)%](P<0.05),空白对照组高于siRNA-MMP-11组(P<0.05)。结论MMP-11通过抑制E-cadherin表达而参与调控骨肉瘤细胞上皮-间质转化,促进骨肉瘤细胞增殖、侵袭和转移。Objective To observe the influences of matrix metalloproteinase-11(MMP-11)on the proliferation,invasion and metastasis of osteosarcoma cells,and to explore its role in regulating epithelial-mesenchymal transition(EMT)of osteosarcoma cells.Methods The Saos-2 cells in logarithmic growth phase were randomly divided into blank control group(normal culture),siRNA-MMP-11 group(transfected with siRNA-MMP-11)and ovRNA-MMP-11 group(transfected with ovRNA-MMP-11).After 48-h transfection,the relative expression of MMP-11 mRNA was detected by real-time fluorescence quantitative PCR,the relative expressions of E-cadherin,N-cadherin and MMP-11 proteins were detected by Western blot,the number of invasive cells was detected by Transwell assay,and the number of migrating cells was detected by scratch test.MTT assay was used to detect the cell proliferation rate of three groups after 72-h culture.Results After 48-h transfection,the relative expressions of MMP-11 mRNA and protein were higher in ovRNA-MMP-11 group(1.511±0.082,1.688±0.115)than those in blank control group(1.339±0.211,1.024±0.066)and siRNA-MMP-11 group(1.147±0.382,0.382±0.068)(P<0.05),and higher in blank control group than those in siRNA-MMP-11 group(P<0.05).The numbers of migrating cells and invasive cells were larger in ovRNA-MMP-11 group(35.528±9.241,352.807±63.715)than those in siRNA-MMP-11 group(21.330±1.627,174.265±50.242)and blank control group(12.330±0.577,335.421±140.261)(P<0.05),the number of migrating cells was larger in siRNA-MMP-11group than that in blank control group(P<0.05),and the number of invasive cells was smaller in siRNA-MMP-11group than that in blank control group(P<0.05).The relative expression of E-cadherin protein was lower in ovRNA-MMP-11group(0.557±0.236)than that in blank control group(0.653±0.202)and siRNA-MMP-11group(0.905±0.324)(P<0.05),and lower in blank control group than that in siRNA-MMP-11group(P<0.05).There was no significant difference in the relative expression of N-cadherin protein among three groups(P>0.05).Aft

关 键 词：骨肉瘤 基质金属蛋白酶-11 上皮-间质转化 转移 SAOS-2细胞 

分 类 号：R738.1[医药卫生—肿瘤]